PMID: 8599179Oct 1, 1995Paper

An activator of blood coagulation factor X from the venom of Bungarus fasciatus

Toxicon : Official Journal of the International Society on Toxinology
Y ZhangC Bon

Abstract

A specific activator of blood coagulation factor X was purified from the venom of Bungarus fasciatus by gel filtration and by ion-exchange chromatography on a Mono-Q column (FPLC). It consisted of a single polypeptide chain, with a mol. wt of 70,000 in reducing and non-reducing conditions. The enzyme had an amidolytic activity towards the chromogenic substrates S-2266 and S-2302 but it did not hydrolyse S-2238, S2251 or S-2222, which are specific substrates for thrombin, plasmin and factor Xa, respectively. The enzyme activated factor X in vitro and the effect was Ca2+ dependent with a Hill coefficient of 7.9. As with physiological activators, the venom activator cleaves the heavy chain of factor X, producing the activated factor Xa alpha. The purified factor X activator from B. fasciatus venom did not activate prothrombin, nor did it cleave or clot purified fibrinogen. The amidolytic activity and the factor X activation activity of the factor X activator from B. fasciatus venom were readily inhibited by serine protease inhibitors such as diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), benzamidine and by soybean trypsin inhibitor but not by EDTA. These observations suggest that the factor X activator f...Continue Reading

References

Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·S A PrestonJ B Harris
Jan 1, 1987·Toxicon : Official Journal of the International Society on Toxinology·A Lôbo de Araújo, F Radvanyi

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Citations

Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·Q B LiT Kosugi
Dec 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·Steven D Aird
May 30, 1998·Toxicon : Official Journal of the International Society on Toxinology·Y ZhangS W Zhu
Dec 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·F S Markland
Aug 2, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mari SamelJüri Siigur
Aug 26, 2014·Journal of Proteomics·Muhamad Rusdi Ahmad RusmiliIekhsan Othman
Apr 28, 2009·Toxicon : Official Journal of the International Society on Toxinology·Ji-Fu WeiShao-Heng He
Feb 1, 2011·Toxicon : Official Journal of the International Society on Toxinology·Tamara SajevicIgor Križaj
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Yang JinYu-Liang Xiong
Sep 29, 2019·Toxins·Tarek Mohamed Abd El-AzizJames D Stockand
Sep 26, 2015·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Sami U Khan, Saad S Al-Saleh
Sep 16, 2018·Toxins·Alex ChapeaurougeStephen P Mackessy
Jul 13, 2017·Revista Da Sociedade Brasileira De Medicina Tropical·Fabiana da Rocha OliveiraJorge Luis Lopez Lozano

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