An Active Type I-E CRISPR-Cas System Identified in Streptomyces avermitilis

PloS One
Yi QiuYuan Song

Abstract

CRISPR-Cas systems, the small RNA-dependent immune systems, are widely distributed in prokaryotes. However, only a small proportion of CRISPR-Cas systems have been identified to be active in bacteria. In this work, a naturally active type I-E CRISPR-Cas system was found in Streptomyces avermitilis. The system shares many common genetic features with the type I-E system of Escherichia coli, and meanwhile shows unique characteristics. It not only degrades plasmid DNA with target protospacers, but also acquires new spacers from the target plasmid DNA. The naive features of spacer acquisition in the type I-E system of S. avermitilis were investigated and a completely conserved PAM 5'-AAG-3' was identified. Spacer acquisition displayed differential strand bias upstream and downstream of the priming spacer, and irregular integrations of new spacers were observed. In addition, introduction of this system into host conferred phage resistance to some extent. This study will give new insights into adaptation mechanism of the type I-E systems in vivo, and meanwhile provide theoretical foundation for applying this system on the genetic modification of S. avermitilis.

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Citations

Apr 6, 2018·International Journal of Molecular Sciences·Suhyung ChoByung-Kwan Cho
Dec 19, 2016·F1000Research·Keith F Chater
Dec 15, 2018·Natural Product Reports·Yaojun TongSang Yup Lee
Jul 22, 2018·Interdisciplinary Sciences, Computational Life Sciences·Jinqi ZhangHong-Yu Ou
Oct 31, 2021·Applied Microbiology and Biotechnology·Carlos Caicedo-MontoyaSergio Sanchez

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Datasets Mentioned

BETA
GM041182

Methods Mentioned

BETA
PCR
PCRs
electrophoresis

Software Mentioned

WebLogo
DNAMAN
Weblogo Basic
BLAST

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