An actively retrotransposing, novel subfamily of mouse L1 elements

The EMBO Journal
T P NaasH H Kazazian

Abstract

Retrotransposition of LINEs and other retroelements increases repetition in mammalian genomes and can cause deleterious mutations. Recent insertions of two full-length L1s, L1spa and L1Orl, caused the disease phenotypes of the spastic and Orleans reeler mice respectively. Here we show that these two recently retrotransposed L1s are nearly identical in sequence, have two open reading frames and belong to a novel subfamily related to the ancient F subfamily. We have named this new subfamily TF (for transposable) and show that many full-length members of this family are present in the mouse genome. The TF 5' untranslated region has promoter activity, and TF-type RNA is abundant in cytoplasmic ribonucleoprotein particles, which are likely intermediates in retrotransposition. Both L1spa and L1Orl have reverse transcriptase activity in a yeast-based assay and retrotranspose at high frequency in cultured cells. Together, our data indicate that the TF subfamily of L1s contains a major class of mobile elements that is expanding in the mouse genome.

References

Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D M SeverynseM H Edgell
Oct 18, 1991·Cell·L K DerrD J Garfinkel
Dec 30, 1991·Science·B A DombroskiH H Kazazian
Dec 30, 1991·Science·S L MathiasA Gabriel
Dec 8, 1987·Biochimica Et Biophysica Acta·T G Fanning, M F Singer
Feb 10, 1995·The Journal of Biological Chemistry·V O Kolosha, S L Martin
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·N B AdeyC A Hutchison
Nov 29, 1996·Cell·J V MoranH H Kazazian
May 1, 1997·Nature Genetics·J D Boeke
May 1, 1997·Nature Genetics·D M SassamanH H Kazazian
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·V O Kolosha, S L Martin

❮ Previous
Next ❯

Citations

Oct 17, 2002·The Anatomical Record·Reid F Thompson, George M Langford
Sep 9, 2010·Theory in Biosciences = Theorie in Den Biowissenschaften·Hugh R MacMillan, Michael J McConnell
Dec 31, 2003·Trends in Genetics : TIG·Anthony V FuranoStephane Boissinot
Jul 5, 2001·Mechanisms of Ageing and Development·S L MartinJ Vijg
Jul 17, 1998·Current Opinion in Structural Biology·J Jurka
May 21, 2004·Nature·Jeffrey S Han, Jef D Boeke
May 20, 1998·Nature Genetics·H H Kazazian, J V Moran
Nov 5, 2002·Nature Genetics·Eric M OstertagHaig H Kazazian
Jan 29, 2013·Nature Structural & Molecular Biology·Anas FadlounMaria-Elena Torres-Padilla
Sep 3, 2013·Nature Structural & Molecular Biology·Sara R HerasJavier F Cáceres
May 27, 2011·Molecular Biology and Evolution·Sergio Lukic, Kevin Chen
Feb 24, 2000·Nucleic Acids Research·E M OstertagH H Kazazian
Aug 9, 2003·Nucleic Acids Research·Nuo YangHaig H Kazazian
Sep 20, 2005·Nucleic Acids Research·Lori H ConlanMarlene Belfort
Feb 7, 2001·Molecular Biology and Evolution·I BusseauA Bucheton
Nov 9, 2000·Molecular Biology and Evolution·M W Simmen, A Bird
Mar 31, 2000·Molecular Biology and Evolution·S C HardiesS Huang
Jun 1, 2000·Molecular Biology and Evolution·S BoissinotA V Furano
Jan 19, 2006·Genes & Development·Reid S AlischJohn V Moran
Oct 10, 2001·Genome Research·J L GoodierH H Kazazian
Sep 6, 2002·Genome Research·Astrid M Roy-EngelPrescott L Deininger
Feb 7, 2001·Molecular and Cellular Biology·W WeiJ V Moran
Aug 1, 2006·Journal of Biomedicine & Biotechnology·Shane R HormanEline T Luning Prak
Aug 1, 2006·Journal of Biomedicine & Biotechnology·Sandra L Martin
Aug 12, 2005·Cytogenetic and Genome Research·M Dewannieux, T Heidmann
Aug 12, 2005·Cytogenetic and Genome Research·S L MartinS Boissinot
Aug 12, 2005·Cytogenetic and Genome Research·N ZinglerG G Schumann
Aug 12, 2005·Cytogenetic and Genome Research·R A GrahnH A Wichman
Feb 18, 2006·Cytogenetic and Genome Research·L A ScottH A Wichman
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·David A Largaespada
Feb 16, 2011·Mobile DNA·Wenfeng AnJef D Boeke
Dec 6, 2007·Genome Biology·Eric M OstertagHiroki Kano
Oct 30, 2008·Genetics·Chun DongJeffrey S Han
Jun 27, 2014·PLoS Genetics·Lei YangHolly Wichman
Nov 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Wenfeng AnJef D Boeke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.