An actuated pump on-chip powered by cultured cardiomyocytes

Lab on a Chip
Yo TanakaT Kitamori

Abstract

Cellular functions are frequently exploited as processing components for integrated chemical systems such as biochemical reactors and bioassay systems. Here, we have created a new cell-based microsystem exploiting the intrinsic pulsatile mechanical functions of cardiomyocytes to build a cellular micropump on-chip using cardiomyocyte sheets as prototype bio-microactuators. We first demonstrate cell-based control of fluid motion in a model microchannel without check valves and evaluate the potential performance of the bio-actuation. For this purpose, a poly(dimethylsiloxane) (PDMS) microchip with a microchannel equipped with a diaphragm and a push-bar structure capable of harnessing collective cell fluid mechanical forces was coupled to a cultured pulsating cardiomyocyte sheet, activating cell-based fluid movement in the microchannel by actuating the diaphragm. Cell oscillation frequency and correlated fluid displacement in this system depended on temperature. When culture temperature was increased, collective cell contraction frequency remained cooperative and synchronous but increased, while displacement was slightly reduced. We then demonstrated directional fluid pumping within microchannels using cantilever-type micro-check v...Continue Reading

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Citations

Oct 22, 2008·Analytical and Bioanalytical Chemistry·Kae SatoTakehiko Kitamori
Jun 19, 2009·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Ken-ichi YamasakiToshia Fujisato
Sep 6, 2013·Annals of Biomedical Engineering·Roger D Kamm, Rashid Bashir
Nov 28, 2009·Biomedical Microdevices·Kazunori ShimizuEiji Nagamori
Sep 5, 2012·Biomedical Microdevices·Yoshitake AkiyamaKeisuke Morishima
Oct 12, 2012·Biomedical Microdevices·Takayuki HoshinoKeisuke Morishima
Jan 12, 2010·In Vitro Cellular & Developmental Biology. Animal·Yoshitake AkiyamaKeisuke Morishima
Dec 10, 2013·Biomaterials·Marine VerhulselJean-Louis Viovy
May 8, 2013·Lab on a Chip·William J PolacheckRoger D Kamm
Mar 21, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yo TanakaTakehiko Kitamori
Dec 15, 2010·International Journal of Molecular Sciences·Hirokazu AkiyamaMasamichi Kamihira
May 8, 2010·Lab on a Chip·Moeto NagaiHiroyuki Fujita
Aug 20, 2011·Lab on a Chip·Aitor EzkerraJesús Miguel Ruano-López
Jul 6, 2013·Lab on a Chip·John P WikswoWilliam J Matloff
Aug 16, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Piyush BajajRashid Bashir
Sep 25, 2012·Biochemical and Biophysical Research Communications·Takayuki HoshinoKeisuke Morishima
Dec 9, 2015·Annual Review of Biomedical Engineering·Adam W Feinberg
Sep 21, 2007·Biosensors & Bioelectronics·Yo TanakaTakehiko Kitamori
Oct 30, 2012·AIChE Journal·Halil TekinAli Khademhosseini
Jun 5, 2014·Small·Rika Wright Carlsen, Metin Sitti
Feb 10, 2010·IEEE Transactions on Nanobioscience·Hiroshi HoriguchiKeisuke Morishima
Jun 27, 2015·Small·Kwanoh KimD L Fan
Sep 2, 2014·Progress in Biophysics and Molecular Biology·Sebastien G M UzelRoger D Kamm
Feb 3, 2007·Lab on a Chip·Yo TanakaTakehiko Kitamori
Feb 12, 2009·Lab on a Chip·Yoshitake AkiyamaKeisuke Morishima
Dec 19, 2013·Lab on a Chip·Vincent ChanRashid Bashir
May 16, 2014·Chemical Reviews·Maria GuixArben Merkoçi
Sep 21, 2016·Biomedical Microdevices·V N Lad, Swati Ralekar
Aug 11, 2017·Biomedical Microdevices·Angelina V ShutkoKonstantin I Agladze
Mar 7, 2017·Bioinspiration & Biomimetics·Leonardo Ricotti, Toshinori Fujie
Oct 4, 2018·Lab on a Chip·Jia Ming ZhangHuiling Duan
Dec 20, 2014·Journal of Laboratory Automation·Joohyung LeeJungkyu Jay Kim
Sep 27, 2007·Lab on a Chip·Jungyul ParkByungkyu Kim

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