An acute dose of gamma-hydroxybutyric acid alters gene expression in multiple mouse brain regions

Neuroscience
B J SchnackenbergT A Patterson

Abstract

Gamma-hydroxybutyric acid (GHB) is normally found in the brain in low concentrations and may function as a neurotransmitter, although the mechanism of action has not been completely elucidated. GHB has been used as a general anesthetic and is currently used to treat narcolepsy and alcoholism. Recreational use of GHB is primarily as a "club drug" and a "date rape drug," due to its amnesic effects. For this study, the hypothesis was that behavioral and neurochemical alterations may parallel gene expression changes in the brain after GHB administration. Adult male C57/B6N mice (n=5/group) were administered a single dose of 500 mg/kg GHB (i.p.) and were sacrificed 1, 2 and 4 h after treatment. Control mice were administered saline. Brains were removed and regionally dissected on ice. Total RNA from the hippocampus, cortex and striatum was extracted, amplified and labeled. Gene expression was evaluated using Agilent whole mouse genome 4x44K oligonucleotide microarrays. Microarray data were analyzed by ArrayTrack and differentially expressed genes (DEGs) were identified using P < or = 0.01 and a fold change > or = 1.7 as the criteria for significance. Principal component analysis (PCA) and Hierarchical Cluster Analysis (HCA) showed t...Continue Reading

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Citations

Feb 22, 2012·Neuroscience and Biobehavioral Reviews·Jan G C van AmsterdamRaymond J M Niesink
Jan 8, 2016·Medicinal Research Reviews·Michel MaitreAyikoe G Mensah-Nyagan
Sep 6, 2011·Neuroscience Letters·Kirsten A Porter-StranskyBrandon J Aragona
Aug 18, 2020·Journal of Alzheimer's Disease : JAD·Bianca Maria CiminelliPatrizia Malaspina
Sep 8, 2016·Alzheimer's Research & Therapy·Michel MaitreAyikoe G Mensah-Nyagan

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