An Acute Mouse Spinal Cord Slice Preparation for Studying Glial Activation ex vivo

Bio-protocol
Juan Mauricio GarréMichael V L Bennett

Abstract

Pathological conditions such as amyotrophic lateral sclerosis, spinal cord injury and chronic pain are characterized by activation of astrocytes and microglia in spinal cord and have been modeled in rodents. In vivo imaging at cellular level in these animal models is limited due to the spinal cord's highly myelinated funiculi. The preparation of acute slices may offer an alternative and valuable strategy to collect structural and functional information in vitro from dorsal, lateral and ventral regions of spinal cord. Here, we describe a procedure for preparing acute slices from mouse spinal cord (Garré et al., 2016). This preparation should allow further understanding of how glial cells in spinal cord respond acutely to various inflammatory challenges.

References

Sep 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jorge E ContrerasMichael V L Bennett
May 17, 2005·Nature Neuroscience·Dimitrios DavalosWen-Biao Gan
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Juan M GarréVerónica Abudara
Oct 28, 2011·Journal of Neurophysiology·Pratip Mitra, Robert M Brownstone
Nov 26, 2013·Glia·Takahiro TakanoMaiken Nedergaard
Apr 29, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Juan Mauricio GarréMichael V L Bennett

❮ Previous
Next ❯

Citations

Mar 20, 2018·Bio-protocol·Thomas Papouin, Philip G Haydon
Mar 29, 2021·The Journal of Pain : Official Journal of the American Pain Society·Jonathan Paulo AgnesAlfeu Zanotto-Filho

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.

ALS: Pathogenic Mechanisms

Amyotrophic Lateral Sclerosis is a progressive neurodegenerative disorder characterized by muscle weakness. Here is the latest research investigating pathogenic mechanisms that underlie this genetically heterogeneous disorder.

Related Papers

Advances in Neurology
H Mitsumoto, E P Pioro
Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology
Andrea CalvoAdriano Chio
Neurosciences : the Official Journal of the Pan Arab Union of Neurological Sciences
Nura H Alkali, Anthony H Pullen
© 2022 Meta ULC. All rights reserved