An acyl-CoA synthase (acoas) gene adjacent to the mycocerosic acid synthase (mas) locus is necessary for mycocerosyl lipid synthesis in Mycobacterium tuberculosis var. bovis BCG.

The Journal of Biological Chemistry
A M Fitzmaurice, Pappachan E Kolattukudy

Abstract

An open reading frame, ORF3, first identified adjacent to the mycocerosic acid synthase gene in Mycobacterium bovis BCG encodes a protein with acyl-CoA synthase (ACoAS) activity. Genes homologous to acoas are found adjacent to other multifunctional polyketide synthase genes in the mycobacterial genome. To test whether these gene products are necessary to esterify the fatty acids generated by the adjacent polyketide synthase gene products, the acoas gene was disrupted in M. bovis BCG using a suicide vector containing the acoas gene with an internal deletion and the hygromycin-resistant gene as selection marker. Allelic exchange at the acoas locus was confirmed by Southern hybridization and polymerase chain reaction amplification of both flanking regions expected from homologous recombination. Immunoblot analysis indicated that the 65-kDa ACoAS protein product was absent in the mutant. Chromatographic analysis of lipids derived from [1-14C]propionate showed that the mutant did not produce mycocerosyl lipids, although it produced normal levels of mycocerosic acid synthase. These results suggest that ACoAS is involved in the synthesis of mycocerosyl lipids of the mycobacterial cell wall.

References

Aug 21, 1992·Science·B R Bloom, C J Murray
Jan 11, 1991·Methods in Enzymology·W R JacobsB R Bloom
Sep 1, 1991·Reviews of Infectious Diseases·F M Collins
Apr 26, 1989·Biochimica Et Biophysica Acta·M Daffé, M A Lanéelle
Jul 1, 1988·Journal of General Microbiology·M Daffé, M A Laneelle
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·J M ReyratB Gicquel
Jan 1, 1995·Annual Review of Biochemistry·P J Brennan, H Nikaido
Jan 1, 1993·Journal of Bacteriology·D B Young, S T Cole
Jan 1, 1996·Journal of Bacteriology·V BalasubramanianW R Jacobs
Mar 1, 1996·Infection and Immunity·M J Fenton, M W Vermeulen
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·A K AzadP E Kolattukudy
Apr 1, 1997·Molecular Microbiology·P E KolattukudyT D Sirakova
Feb 28, 1998·The Journal of Biological Chemistry·N D Fernandes, P E Kolattukudy

❮ Previous
Next ❯

Citations

Sep 25, 2004·Medicinal Research Reviews·Rama P TripathiVinod K Tiwari
Jan 26, 2005·Molecular Genetics and Genomics : MGG·A Rao, A Ranganathan
Jul 9, 1999·Progress in Lipid Research·C C DiRussoJ D Weimar
Oct 22, 2003·FEMS Microbiology Letters·Amit SinghAnil K Tyagi
Oct 26, 2000·Current Opinion in Microbiology·J E SheaR G Feldman
Apr 4, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Aneesh GoyalRajan Sankaranarayanan
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Eckhart Schweizer, Jörg Hofmann
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kenolisa C OnwuemeLuis E N Quadri
Mar 18, 2006·Tuberculosis·R Hernàndez PandoB Gicquel
Mar 15, 2014·Critical Reviews in Biochemistry and Molecular Biology·Luis E N Quadri
May 29, 2002·Chemistry & Biology·David E MinnikinGurdyal S Besra
Jul 12, 2017·ACS Chemical Biology·Danielle D BarnesEoin M Scanlan
Jan 7, 2015·Journal of Bacteriology·Olivia VergnolleLuis E N Quadri
Oct 8, 2005·The Journal of Biological Chemistry·Gustavo StadthagenMary Jackson
Jun 25, 2015·Microbiology Spectrum·Mamadou DafféMary Jackson
Aug 24, 2005·Progress in Lipid Research·Kenolisa C OnwuemeLuis E N Quadri

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.