An adaptable chromosome preparation methodology for use in invertebrate research organisms

BMC Biology
Longhua GuoAlejandro Sánchez Alvarado

Abstract

The ability to efficiently visualize and manipulate chromosomes is fundamental to understanding the genome architecture of organisms. Conventional chromosome preparation protocols developed for mammalian cells and those relying on species-specific conditions are not suitable for many invertebrates. Hence, a simple and inexpensive chromosome preparation protocol, adaptable to multiple invertebrate species, is needed. We optimized a chromosome preparation protocol and applied it to several planarian species (phylum Platyhelminthes), the freshwater apple snail Pomacea canaliculata (phylum Mollusca), and the starlet sea anemone Nematostella vectensis (phylum Cnidaria). We demonstrated that both mitotically active adult tissues and embryos can be used as sources of metaphase chromosomes, expanding the potential use of this technique to invertebrates lacking cell lines and/or with limited access to the complete life cycle. Simple hypotonic treatment with deionized water was sufficient for karyotyping; growing cells in culture was not necessary. The obtained karyotypes allowed the identification of differences in ploidy and chromosome architecture among otherwise morphologically indistinguishable organisms, as in the case of a mixed p...Continue Reading

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Aug 16, 2017·Nature Ecology & Evolution·Longhua GuoAlejandro Sánchez Alvarado

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Citations

Jan 26, 2021·STAR Protocols·Paul G Barghouth, Néstor J Oviedo
Jul 13, 2021·PLoS Genetics·Katya L MackSuzanne E McGaugh
Sep 22, 2021·G3 : Genes - Genomes - Genetics·Darrin T SchultzRichard E Green
Jan 12, 2021··Paul G. Barghouth, Néstor J. Oviedo

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