An adaptable, portable microarray reader for biodetection.

Sensors
Deanna L ThompsonMatthew A Coleman

Abstract

We have developed an inexpensive portable microarray reader that can be applied to standard microscope slide-based arrays and other array formats printed on chemically modified surfaces. Measuring only 19 cm in length, the imaging device is portable and may be applicable to both triage and clinical settings. For multiplexing and adaptability purposes, it can be modified to work with multiple excitation/emission wavelengths. Our device is shown to be comparable to a commercial laser scanner when detecting both streptavidin-biotin and antibody interactions. This paper presents the development and characterization of a handheld microarray imager and directly compares it with a commercial scanner.

References

Nov 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·J J ShawR Lainson
Feb 9, 1999·Analytical Chemistry·T Vo-DinhM N Ericson
May 18, 1999·Analytical Biochemistry·A LuekingG Walter
Aug 19, 2000·Electrophoresis·T O JoosH Hämmerle
Feb 7, 2001·Applied and Environmental Microbiology·S G BavykinA D Mirzabekov
Jan 16, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Reinhard HillerRudolf Valenta
Feb 6, 2002·Genome Research·Ajay N JainDaniel Pinkel
Sep 14, 2004·Journal of Proteome Research·Rupa S RaoMatthew A Coleman
Jan 27, 2005·Molecular & Cellular Proteomics : MCP·Brian B Haab

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Citations

Apr 25, 2013·Biosensors & Bioelectronics·P KozmaF F Bier
Dec 29, 2009·Analytical Biochemistry·Yury M ShlyapnikovVictor N Morozov
Apr 26, 2016·Sensors·Palmiro PoltronieriIda Barbara Reca
Sep 2, 2014·Foods·Palmiro PoltronieriGiuseppe Maruccio

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Methods Mentioned

BETA
biosensor
chip
Protein Arraying
Protein Array

Software Mentioned

UCSF Spot
Excel
Image J
ImageJ
MATLAB
Image Processing Toolbox

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