An additional promoter within the protein-coding region of the psbD-psbC gene cluster in tobacco chloroplast DNA.

Nucleic Acids Research
W B YaoMasahiro Sugiura

Abstract

Transcription of the psbD-psbC gene cluster in tobacco chloroplasts has been studied. This cluster contains in linear sequence the overlapping genes encoding the D2 and 43 kDa proteins of Photosystem II (psbD and psbC, respectively), and ORF62. Eight major transcripts ranging from 1.5 to 4.4 kb were detected by northern blot analysis. S1 mapping experiments revealed that these multiple transcripts comprise five distinct 5' ends whose precise positions were further determined by primer extension analysis. Two of the five 5' ends were determined to be the transcriptional initiation sites by in vitro capping assays: the main site is located 905 bp upstream from the ATG codon of psbD and the additional site is 194 bp upstream from the first ATG codon of psbC. The latter site and the preceding prokaryotic promoter motif are within the protein-coding region of psbD. The 3' ends of transcripts were determined by S1 mapping.

Citations

Aug 28, 2020·International Journal of Molecular Sciences·Yi ZhangCongming Lu
Jul 29, 2017·Frontiers in Plant Science·Shuichi ShimmuraTakashi Shiina
Feb 3, 1999·Annual Review of Genetics·M SugiuraM Sugita
Dec 6, 2011·Nucleic Acids Research·Hannes Ruwe, Christian Schmitz-Linneweber
Mar 8, 2020·Plants·Piotr GawrońskiLars B Scharff
Mar 22, 2001·The Journal of Biological Chemistry·E Baena-GonzalezP Maenpaa
Oct 1, 1993·Molecular & General Genetics : MGG·J C Tonkyn, W Gruissem
Oct 1, 1996·Plant Molecular Biology·M Sugita, M Sugiura
Sep 10, 2017·Plant Molecular Biology·Marie-Kristin LehnigerChristian Schmitz-Linneweber
Feb 1, 1993·Current Genetics·A Kanno, A Hirai
Apr 2, 2021·Plant Physiology·Han-Yi FuMark Aurel Schöttler

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