An adenosine A3 receptor agonist inhibits DSS-induced colitis in mice through modulation of the NF-κB signaling pathway

Scientific Reports
Tianhua RenYu Zhou

Abstract

The role of the adenosine A3 receptor (A3AR) in experimental colitis is controversial. The A3AR agonist N(6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA) has been shown to have a clinical benefit, although studies in A3AR-deficient mice suggest a pro-inflammatory role. However, there are no studies on the effect of 2-Cl-IB-MECA and the molecular mechanism of action of A3AR in murine colitis models in vivo. Is it the same as that observed in vitro? The interaction between 2-CL-IB-MECA and A3AR in a murine colitis model and the signaling pathways associated with this interaction remain unclear. Here we demonstrate a role for the NF-κB signaling pathway and its effect on modifying the activity of proinflammatory factors in A3AR-mediated biological processes. Our results demonstrated that A3AR activation possessed marked effects on experimental colitis through the NF-κB signaling pathway.

References

Sep 19, 2007·Expert Opinion on Emerging Drugs·Zhan-Guo Gao, Kenneth A Jacobson
May 16, 2008·Journal of Internal Medicine·I AtreyaM F Neurath
Jun 10, 2008·British Journal of Pharmacology·Vl KolachalaSv Sitaraman
Jul 8, 2008·Gastroenterology·Vasantha L KolachalaShanthi V Sitaraman
Sep 2, 2008·Nature Reviews. Drug Discovery·György HaskóPál Pacher
Jun 17, 2009·Current Opinion in Gastroenterology·Martina E Spehlmann, Lars Eckmann
Jul 30, 2009·Handbook of Experimental Pharmacology·P A BoreaP Fishman
Sep 25, 2009·World Journal of Gastroenterology : WJG·Jeff Huaqing Ye, Vazhaikkurichi M Rajendran
Dec 15, 2010·Cell Research·Andy WullaertManolis Pasparakis
Dec 17, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Fang YanD Brent Polk
May 19, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lesley RheeDavid L Boone
Jul 3, 2013·Biochemical and Biophysical Research Communications·Hyung-Jun LeeHye-Kyung Na
Nov 14, 2013·Cardiovascular Drugs and Therapy·Afthab HussainHelen L Maddock
Jan 31, 2014·Journal of Cellular Physiology·Seyed Mahdi HassanianAlireza R Rezaie

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Citations

Apr 12, 2017·The Journal of Pharmacy and Pharmacology·Jan Schmidt, Polonca Ferk
Mar 9, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vinay KumarMahesh Mohan
Jul 1, 2017·Journal of Cellular Physiology·Amirhossein BahreyniSeyed M Hassanian
Jan 24, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jessica L BowserHolger K Eltzschig
Oct 19, 2019·Cancers·Simone de LeveVerena Jendrossek
Mar 28, 2020·Cells·Wiwin Is EffendiYoshihiro Nishimura
May 19, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Geng-Hao LiuTzung-Yan Lee
Jul 28, 2020·International Journal of Molecular Sciences·Vanessa D'AntongiovanniLuca Antonioli
Nov 9, 2020·Purinergic Signalling·Stefania Giotti CioatoIraci L S Torres
Jul 25, 2021·International Journal of Molecular Sciences·Silvia PasquiniKatia Varani

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Methods Mentioned

BETA
nuclear translocation
biopsies
enzyme-linked immunosorbent assay
protein assay
electrophoresis
Reverse Transcription
PCR

Software Mentioned

Quantity One

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