An Adult Zebrafish Model Reveals that Mucormycosis Induces Apoptosis of Infected Macrophages

Scientific Reports
Azucena López-MuñozVictoriano Mulero

Abstract

Mucormycosis is a life-threatening fungal infection caused by various ubiquitous filamentous fungi of the Mucorales order, although Rhizopus spp. and Mucor spp. are the most prevalent causal agents. The limited therapeutic options available together with a rapid progression of the infection and a difficult early diagnosis produce high mortality. Here, we developed an adult zebrafish model of Mucor circinelloides infection which allowed us to confirm the link between sporangiospore size and virulence. Transcriptomic studies revealed a local, strong inflammatory response of the host elicited after sporangiospore germination and mycelial tissue invasion, while avirulent and UV-killed sporangiospores failed to induce inflammation and were rapidly cleared. Of the 857 genes modulated by the infection, those encoding cytokines, complement factors, peptidoglycan recognition proteins, and iron acquisition are particularly interesting. Furthermore, neutrophils and macrophages were similarly recruited independently of sporangiospore virulence and viability, which results in a robust depletion of both cell types in the hematopoietic compartment. Strikingly, our model also reveals for the first time the ability of mucormycosis to induce the...Continue Reading

References

Feb 7, 2001·Clinical Microbiology Reviews·J A RibesD J Baker
Jan 25, 2005·Infection and Immunity·Ashraf S IbrahimJohn E Edwards
Aug 5, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Maureen M RodenThomas J Walsh
Mar 29, 2006·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M ChayakulkeereeJ R Perfect
Aug 24, 2006·Blood·Stephen A RenshawMoira K B Whyte
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Iván MuleroVictoriano Mulero
Jun 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Georgios ChamilosDimitrios P Kontoyiannis
Dec 22, 2009·Bioinformatics·Bo LiColin N Dewey
Nov 19, 2010·Blood·Felix EllettGraham J Lieschke
Apr 8, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·David TaylorNicole Meissner
Jun 24, 2011·PLoS Pathogens·Charles H LiSoo Chan Lee
Jan 25, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·George PetrikkosDimitrios P Kontoyiannis
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Mar 21, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Fanny LanternierOlivier Lortholary
Jun 20, 2012·Methods in Molecular Biology·Santiago Torres-MartínezAna Vila
Dec 12, 2012·The New England Journal of Medicine·Robyn Neblett FanfairBenjamin J Park
Dec 21, 2012·Science Translational Medicine·Gordon D BrownTheodore C White
Nov 2, 2013·The Journal of Clinical Investigation·Michail S LionakisPhilip M Murphy
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Nov 8, 2014·Cold Spring Harbor Perspectives in Medicine·Leonel MendozaSoo Chan Lee
Sep 24, 2015·Disease Models & Mechanisms·Kerstin VoelzRobert T Wheeler
Jan 21, 2017·PLoS Pathogens·Trung Anh TrieuFrancisco E Nicolás
Aug 5, 2017·PLoS Pathogens·Clara Baldin, Ashraf S Ibrahim

❮ Previous
Next ❯

Citations

Mar 1, 2019·Medical Mycology·Mohamed I Abdelwahab Hassan, Kerstin Voigt
Feb 11, 2020·BMC Genomics·Annie LebretonLaurence Meslet-Cladière
Mar 20, 2020·Genes·Carlos LaxFrancisco Esteban Nicolás
Apr 3, 2019·Journal of Fungi·Ilse D Jacobsen
Jul 2, 2020·Journal of Fungi·Francisco E NicolásVictoriano Garre
Jul 23, 2020·Frontiers in Genetics·Alexandra Y SoareVincent M Bruno
Dec 16, 2018·Journal of Fungi·Emily E RosowskiJ Muse Davis
Aug 27, 2021·Journal of Fungi·Hariprasath PrakashShivaprakash Mandya Rudramurthy

❮ Previous
Next ❯

Methods Mentioned

BETA
gene
gene knockdown
RNA-seq
PCR

Software Mentioned

Bowtie2
FastQC
edgeR
HTSeq
R
Leica Qwin
Ensembl
Blast2GO
gplots R

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here