An Advanced, Silicon-Based Substrate for Sensitive Nucleic Acids Detection

Sensors
Salvatore PetraliaSabrina Conoci

Abstract

Surface substrate and chemical functionalization are crucial aspects for the fabrication of the sensitive biosensor based on microarray technology. In this paper, an advanced, silicon-based substrate (A-MA) allowing enhancement of optical signal for microarray application is described. The substrate consists in a multilayer of Si/Al/SiO₂ layers. The optical signal enhancement is reached by a combination of the mirror effect of Al film and the SiO₂ thickness around 830 nm, which is able to reach the maximum of interference for the emission wavelength of the Cy5 fluorescent label. Moreover, SiO₂ layer is suitable for the immobilization of single-strand DNA through standard silane chemistry, and probe densities of about 2000 F/um² are reached. The microarray is investigated in the detection of HBV (Hepatitis B Virus) pathogen with analytical samples, resulting in a dynamic linear range of 0.05⁻0.5 nM, a sensitivity of about 18000 a.u. nM-1, and a Limit of Detection in the range of 0.031⁻0.043 Nm as a function of the capture probe sequence.

References

Jan 23, 1999·Nature Genetics·P O Brown, D Botstein
Aug 3, 2001·Nature Cell Biology·A Schulze, J Downward
Jan 29, 2002·Nucleic Acids Research·A W PetersonR M Georgiadis
Jul 16, 2002·Annual Review of Biomedical Engineering·Michael J Heller
Dec 15, 2005·Analytical Chemistry·Shohei YamamuraAtsushi Muraguchi
Jul 11, 2006·Nucleic Acids Research·Yang GaoRosina M Georgiadis
Dec 22, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Barbara FoglieniLaura Cremonesi
Feb 19, 2013·Expert Review of Proteomics·Jung-Rok LeeShan X Wang
Oct 28, 2016·Analytical Chemistry·Yiliang ZhaoSharon M Weiss
Jul 29, 2017·ACS Sensors·Salvatore Petralia, Sabrina Conoci
Sep 1, 2018·Light, Science & Applications·Carlos García-MecaJavier Martí

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Citations

Sep 12, 2020·Biotechnology Journal·Joyita Sarkar, Ashok Kumar

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Methods Mentioned

BETA
PCR

Software Mentioned

MA
InCheck Platform H - MAT

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