An AFLP-based approach for the identification of sex-linked markers in blunt snout bream, Megalobrama amblycephala (Cyprinidae)

Genetics and Molecular Research : GMR
H O RaoZ-X Gao

Abstract

Sex-specific DNA markers are useful for studying sex-determination mechanisms and establishment of monosex populations. Three widely spaced geographical populations (Liangzi, Poyang and Yuni Lakes in China) of blunt snout bream (Megalobrama amblycephala) were screened with AFLPs to search for sex-linked markers. Female and male pools (10 individuals in each pool) from each population were screened using 64 different primer combinations. A total of 4789 genomic fragments were produced, with a mean frequency of 75 bands per primer pair. Three different primer combinations produced putative sex-associated amplifications and were selected for individual screening in the three populations. However, none showed sex specificity when we converted these three markers into sequence characterized amplified region markers and evaluated all the individuals from the three populations.

References

Nov 11, 1995·Nucleic Acids Research·P VosM Kuiper
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Oct 10, 2001·Genome Research·Z NingJ C Mullikin
Nov 25, 2003·Nature Reviews. Genetics·Gordon LuikartPierre Taberlet
Aug 2, 2005·Nature·Marcel MarguliesJonathan M Rothberg
Feb 20, 2007·Molecular Ecology·Amy Bouck, Todd Vision
Mar 14, 2007·Plant Physiology·Andreas P M WeberJohn B Ohlrogge
Jul 31, 2007·The Plant Journal : for Cell and Molecular Biology·W Brad BarbazukPatrick S Schnable
Feb 13, 2008·Molecular Ecology·J Cristobal VeraJames H Marden
Feb 18, 2009·Animal Genetics·E-S KimB W Kirkpatrick
Apr 30, 2009·Molecular and Cellular Probes·Jianjun ChenZhongjie Chang
Dec 10, 2009·Nature Reviews. Genetics·Michael L Metzker
May 19, 2010·Animal Genetics·M Pérez-Enciso, L Ferretti
Jul 8, 2010·Fish Physiology and Biochemistry·Xiaohua XiaZhongjie Chang
Aug 27, 2010·Journal of Fish Biology·Z X GaoW M Wang
Jun 28, 2011·Molecular Biology Reports·Fu-Hao LuYong-Jin Park

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Citations

May 29, 2015·International Journal of Molecular Sciences·Xiaoke DuanWeimin Wang

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