An Agonistic Antibody to EPHA2 Exhibits Antitumor Effects on Human Melanoma Cells

Anticancer Research
Atsushi SakamotoShigaku Ikeda

Abstract

EPH receptor A2 (EPHA2) is highly expressed in aggressive types of human cancer, and is expected to be an excellent target molecule for antibody treatments. In this study, we investigated the therapeutic potential of antibody to EPHA2 against melanoma in vitro. We generated three monoclonal antibodies (mAbs) to EPHA2 and examined cell-surface expression by flow cytometry. To investigate the ability to inhibit tumor cell migration therapy with mAbs to EPHA2, we performed a wound scratch assay and invasion assay. We investigated the therapeutic effects of immunotoxins consisting of toxin-conjugated EPHA2 mAbs. All human melanoma cell lines studied expressed EPHA2. Like natural ligand ephrin-A1, one of EPHA2 mAbs, SHM16, inhibited metastatic behavior of cells, such as migration and invasion. In addition, drastic growth inhibition and cytotoxicity were found using immunotoxin-conjugated SHM16. These observations indicate a promising role for EPHA2 as a target in antibody treatments for melanoma, and demonstrate the potential therapeutic effects of an agonistic antibody to EPHA2.

Citations

May 28, 2020·Investigative Ophthalmology & Visual Science·Shiwani SharmaMaria C Inacio
Aug 20, 2020·Journal of Hematology & Oncology·Ta XiaoMin Su
Sep 30, 2020·Molecular Biology Reports·Max London, Eugenio Gallo
Jul 8, 2020·Pharmaceuticals·Sarah BaudetXavier Nicol
Mar 10, 2021·Oncogene·Kalin WilsonDana M Brantley-Sieders
May 9, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Ping ZhaoCeshi Chen
Aug 4, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yongping Hao, Guang Li

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