An alkaline active feruloyl-CoA synthetase from soil metagenome as a potential key enzyme for lignin valorization strategies

PloS One
Victoria SodréFabio M Squina

Abstract

Ferulic acid (FA), a low-molecular weight aromatic compound derived from lignin, represents a high-value molecule, used for applications in the cosmetic and pharmaceutical industries. FA can be further enzymatically converted in other commercially interesting molecules, such as vanillin and bioplastics. In several organisms, these transformations often start with a common step of FA activation via CoA-thioesterification, catalyzed by feruloyl-CoA synthetases (Fcs). In this context, these enzymes are of biotechnological interest for conversion of lignin-derived FA into high value chemicals. In this study, we describe the first structural characterization of a prokaryotic Fcs, named FCS1, isolated from a lignin-degrading microbial consortium. The FCS1 optimum pH and temperature were 9 and 37°C, respectively, with Km of 0.12 mM and Vmax of 36.82 U/mg. The circular dichroism spectra indicated a notable secondary structure stability at alkaline pH values and high temperatures. This secondary structure stability corroborates the activity data, which remains high until pH 9. The Small Angle X-Ray Scattering analyses resulted on the tertiary/quaternary structure and the low-resolution envelope in solution of FCS1, which was modeled as ...Continue Reading

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Citations

Dec 17, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Marcelo Vizoná LiberatoFabio Marcio Squina

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Datasets Mentioned

BETA
MG214406
OYW44890.1

Methods Mentioned

BETA
size-exclusion chromatography
electrophoresis
dynamic light scattering
X-ray
Fluorescence
size exclusion chromatography
size-exclusion column
FCS
Circular dichroism
fluorescence spectroscopy

Software Mentioned

HHPRED
Protparam
Coffee
DAMAVER
MODELLER
32Karat
GNOM
FIT2D
DAMMIN
CRYSOL

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