An all-in-one microfluidic device for parallel DNA extraction and gene analysis

Biomedical Microdevices
Y ZhangT H Wang

Abstract

We have developed a microfluidic device capable of fully integrated sample preparation and gene analysis from crude biosamples such as whole blood. Our platform takes the advantage of the silica superparamagnetic particle based solid phase extraction to develop an all-in-one scheme that performs cell lysis, DNA binding, washing, elution and the PCR in the same reaction chamber. The device also employs a unique reagent loading scheme, allowing efficient preparation of multiple reactions via a single injection channel. In addition, PCR is performed in a droplet-in-oil manner, eliminating the need for chamber sealing. The combination of these design features greatly reduces the complexity in implementing fully integrated lab-on-a-chip systems for genetic detection, facilitating parallel analysis of multiple samples or genes on a single microchip. The capability of the device is demonstrated by performing DNA isolation from the human whole blood sample and analyzing the Rsf-1 gene using the TaqMan probe based gene specific PCR assays.

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Citations

Nov 12, 2014·Microfluidics and Nanofluidics·Dong Jin ShinTza-Huei Wang
Dec 12, 2012·Autoimmunity Reviews·Elisa MenegattiDario Roccatello
Jul 10, 2013·Biosensors & Bioelectronics·Chi-Han ChiouTza-Huei Wang
Jul 12, 2011·Biotechnology Advances·Seungkyung ParkSamuel Yang
Jan 1, 2011·Annual Review of Chemical and Biomolecular Engineering·Eric Livak-DahlMark Burns
Dec 20, 2016·ACS Applied Materials & Interfaces·Guoyou HuangFeng Xu
Oct 12, 2013·Journal of Laboratory Automation·Pornpat AthamanolapTza-Huei Wang
Jul 11, 2014·Annals of Biomedical Engineering·Dong Jin Shin, Tza-Huei Wang
Apr 3, 2019·Annual Review of Analytical Chemistry·Dong Jin ShinTza-Huei Wang
May 26, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Aniruddha M KaushikTza-Huei Wang
Apr 30, 2020·Electrophoresis·Sumera KhizarAbdelhamid Elaissari
Oct 13, 2011·Lab on a Chip·Laura PasquardiniCecilia Pederzolli
Jul 21, 2017·Micromachines·Darren W BranchKomandoor E Achyuthan
Mar 30, 2018·Biomedical Microdevices·Alejandro StarkTza-Huei Wang
Nov 9, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Nikolaos Asproulis, Dimitris Drikakis
Nov 17, 2020·Electrophoresis·Benjamin SarnoStuart D Ibsen
Jan 29, 2021·International Journal of Environmental Research and Public Health·Giorgia CarusoTeresa Fasciana
Sep 10, 2019·ACS Applied Materials & Interfaces·Xuemin WangCraig A Aspinwall

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