PMID: 15381848Sep 24, 2004Paper

An allele-specific polymerase chain reaction assay for the differentiation of members of the Anopheles culicifacies complex

Journal of Biosciences
Om P SinghS K Subbarao

Abstract

Anopheles culicifacies, the principal vector of malaria in India, is a complex of five cryptic species which are morphologically indistinguishable at any stage of life. In view of the practical difficulties associated with classical cytotaxonomic method for the identification of members of the complex, an allele-specific polymerase chain reaction (ASPCR) assay targeted to the D3 domain of 28S ribosomal DNA was developed. The assay discriminates An. culicifacies species A and D from species B, C and E. The assay was validated using chromosomally identified specimens of An. culicifacies from different geographical regions of India representing different sympatric associations. The assay correctly differentiates species A and D from species B, C and E. The possible use of this diagnostic assay in disease vector control programmes is discussed.

References

Nov 1, 1992·Transactions of the Royal Society of Tropical Medicine and Hygiene·S K SubbaraoV P Sharma
Feb 1, 1996·Insect Molecular Biology·F H Collins, S M Paskewitz
Mar 16, 2000·Transactions of the Royal Society of Tropical Medicine and Hygiene·T AdakO P Singh
Sep 5, 2002·Annual Review of Entomology·Jaroslaw Krzywinski, Nora J Besansky

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Citations

Oct 9, 2008·Bioinformation·Ranil Samantha DassanayakeBabaranda Dhammacharige Don Nissanka Kolitha De Silva
Jun 18, 2015·Parasites & Vectors·Cherry L DykesOm P Singh
Jan 8, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·S ManguinM Coosemans
Dec 7, 2011·Acta Tropica·Aparup DasNeena Valecha
Jul 22, 2019·Parasites & Vectors·Azzam M AlahmedRalph E Harbach

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