PMID: 9420042Jan 7, 1998Paper

An allelic variant of the chromosomal gene for class A beta-lactamase K2, specific for Klebsiella pneumoniae, is the ancestor of SHV-1

Antimicrobial Agents and Chemotherapy
S HaeggmanL G Burman

Abstract

Fecal Klebsiella isolates from neonates in 22 Swedish special care units were examined by a PCR we developed for detection of the SHV-1 beta-lactamase gene. All 105 K. pneumoniae isolates and all 11 K. pneumoniae reference strains (including the K. pneumoniae subsp. pneumoniae, ozaenae, and rhinoscleromatis type strains) tested were positive, whereas all 67 K. oxytoca isolates and the K. oxytoca, K. planticola, and K. terrigena type strains tested were negative. Resistance to beta-lactams in K. pneumoniae was not transferable by conjugation, and the beta-lactamase gene was never found on a plasmid. Southern blot analysis showed that the gene had a defined chromosomal location. Isoelectric focusing and sequencing of 231-bp PCR amplicons from different isolates revealed many variants of the enzyme, with the two main groups being SHV-1 like (pI 7.6; 68 isolates) and LEN-1 like (pI 7.1; 14 isolates). Clavulanic acid markedly reduced the MICs of ampicillin for all the K. pneumoniae isolates tested. This fact, MIC profiles (penicillin rather than cephalosporin resistance), pIs, and sequence data showed that the chromosomal beta-lactamase of K. pneumoniae is a class A, group 2 enzyme distinct from the chromosomal AmpC enzymes found in...Continue Reading

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Citations

Jan 11, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H-J MonsteinL E Nilsson
Sep 23, 2016·Frontiers in Microbiology·Apostolos LiakopoulosDaniela Ceccarelli
Apr 24, 2004·Canadian Journal of Microbiology·Jan Walther-Rasmussen, Niels Høiby
Apr 19, 2000·Antimicrobial Agents and Chemotherapy·J W TeoC L Poh
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·S HaeggmanS Brisse
Jan 28, 2005·The New England Journal of Medicine·George A Jacoby, Luisa Silvia Munoz-Price
Oct 16, 2015·The Open Microbiology Journal·Ruchir Chavada, Michael Maley
Jan 25, 2017·Antimicrobial Agents and Chemotherapy·Frank R DeLeoBarry N Kreiswirth
Sep 15, 2001·Antimicrobial Agents and Chemotherapy·J ChavesC Ampurdanés
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Dec 12, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M Gniadkowski
Mar 6, 1999·Current Opinion in Microbiology·K Bush, G H Miller
Aug 25, 2006·Journal of Clinical Microbiology·Maria Silvana AlvesBeatriz Meurer Moreira

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