PMID: 9634757Feb 1, 1995Paper

An allosteric hammerhead ribozyme

Bio/technology
H Porta, P M Lizardi

Abstract

We have constructed an RNA molecule containing a hammerhead ribozyme that is under allosteric control. In the inactive state, the RNA enzyme is unable to cleave a suitable substrate. The formation of the active state of the ribozyme is triggered by a specific interaction with a DNA oligonucleotide effector that is complementary to a single-stranded loop in the RNA enzyme molecule. Other DNA or RNA molecules containing unrelated nucleotide sequences do not function as allosteric effectors. This work demonstrates the feasibility of designing RNA enzymes that are specifically activated in response to an artificially designed molecular recognition event. Such enzymes may have practical applications.

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Citations

Sep 1, 2009·Cellular and Molecular Life Sciences : CMLS·Cedric ReymondJean-Pierre Perreault
Jun 11, 2002·Journal of Molecular Biology·Dennis Y WangDipankar Sen
Jun 17, 2000·Journal of Inorganic Biochemistry·J M ZhouK Taira
Nov 11, 1999·Trends in Biotechnology·G A Soukup, R R Breaker
Feb 5, 2003·Current Opinion in Biotechnology·Mats GullbergUlf Landegren
Jun 14, 2000·Current Opinion in Structural Biology·G A Soukup, R R Breaker
Dec 5, 2000·Current Opinion in Chemical Biology·T KuwabaraK Taira
Aug 16, 2000·Journal of Biotechnology·J HesselberthA D Ellington
Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
Oct 26, 2005·Nature Biotechnology·Robert Penchovsky, Ronald R Breaker
Sep 2, 1998·Nucleic Acids Research·J Tang, R R Breaker
Jul 25, 2000·Nucleic Acids Research·M N StojanovicD W Landry
Mar 27, 2001·Nucleic Acids Research·A M JoseR R Breaker
Jun 17, 2004·Nucleic Acids Research·S Hani Najafi-ShoushtariMichael Famulok
Sep 23, 2006·Nucleic Acids Research·Sergey Amontov, Andres Jäschke
May 8, 2013·Journal of Cheminformatics·Effirul Ikhwan Ramlan, Klaus-Peter Zauner
Apr 2, 2004·Biological & Pharmaceutical Bulletin·Yasuo Komatsu
Mar 8, 2005·Organic & Biomolecular Chemistry·Shinsuke SandoYasuhiro Aoyama
Mar 15, 2011·Bio Systems·Effirul I Ramlan, Klaus-Peter Zauner
Jul 6, 2015·Biochimie·Alla PeselisAlexander Serganov
Apr 17, 2003·FEMS Microbiology Reviews·Elena Puerta-FernándezAlfredo Berzal-Herranz
May 31, 2011·Insect Biochemistry and Molecular Biology·Helena PortaAlejandra Bravo
Jan 12, 2008·CNS Spectrums·J Gorman
Dec 6, 2002·BMC Biotechnology·Kristin M ThompsonAndrew D Ellington
Jun 26, 2004·Chemistry & Biology·Karl KossenScott D Seiwert
May 2, 2002·Chemistry & Biology·Matthew Levy, Andrew D Ellington
Oct 1, 2016·Wiley Interdisciplinary Reviews. RNA·Michele Felletti, Jörg S Hartig
Apr 3, 2001·Nature Biotechnology·S SeetharamanR R Breaker
Aug 19, 2000·Nature·B YurkeJ L Neumann
Jul 26, 2008·Nature Nanotechnology·Jonathan Bath, Andrew J Turberfield
Jul 16, 2002·Nature Biotechnology·Narendra K VaishScott D Seiwert
May 24, 2001·Archives of Biochemistry and Biophysics·N DolzhanskayaR B Denman

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