An alpha to beta conformational switch in EF-Tu

Structure
K AbelF Jurnak

Abstract

The bacterial elongation factor EF-Tu recognizes and transports aminoacyl-tRNAs to mRNA-programmed ribosomes. EF-Tu shares many structural and functional properties with other GTPases whose conformations are regulated by guanine nucleotides. An intact form of Escherichia coli EF-Tu complexed with GDP has been crystallized in the presence of the EF-Tu-specific antibiotic GE2270 A. The three-dimensional structure has been solved by X-ray diffraction analysis and refined to a final crystallographic R factor of 17.2% at a resolution of 2.5 A. The location of the GE2270 A antibiotic-binding site could not be identified. The structure of EF-Tu-GDP is nearly identical to that of a trypsin-modified form of EF-Tu-GDP, demonstrating conclusively that the protease treatment had not altered any essential structural features. The present structure represents the first view of an ordered Switch I region in EF-Tu-GDP and reveals similarities with two other GTPases complexed with GDP: Ran and ADP-ribosylation factor-1. A comparison of the Switch I regions of the GTP and GDP forms of EF-Tu also reveals that a segment, six amino acids in length, completely converts from an alpha helix in the GTP complex to beta secondary structure in the GDP for...Continue Reading

Citations

Jul 14, 1998·FEBS Letters·F MansillaB F Clark
Jul 25, 1998·FEBS Letters·J Nyborg, A Liljas
Aug 11, 2000·Biological Chemistry·M SprinzlK Szkaradkiewicz
Feb 17, 2000·The EMBO Journal·P NissenJ Nyborg
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Maithri M K JayasekeraKaren Joy Shaw
May 7, 2009·The Journal of Biological Chemistry·Alexander S Spirin
Dec 26, 2012·European Biophysics Journal : EBJ·Ping Xie
Jan 26, 2013·Proteins·Julia Koehler LemanJens Meiler
Jul 6, 2014·The Journal of Biological Chemistry·Benjamin J BurnettScott C Blanchard
Jun 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sandro F F PereiraJonathan Dworkin
Feb 6, 2017·The Journal of Biological Chemistry·Kevin B DagbayJeanne A Hardy
Jul 16, 2017·Molecular Microbiology·Samantha M PreziosoJoanna B Goldberg
Jul 3, 2020·Nature·Anna B LovelandAndrei A Korostelev
Dec 10, 1999·Folia Microbiologica·B KraalC Kleanthous
Jun 17, 1999·FEMS Microbiology Reviews·M V RodninaW Wintermeyer
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·A B Simonson, J A Lake
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·G R Andersen, J Nyborg
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·S R Sprang
Oct 24, 2003·Protein Science : a Publication of the Protein Society·Kazuyoshi Ikeda, Junichi Higo
Oct 21, 2003·Nature Structural Biology·Mikel ValleJoachim Frank
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth VillaJoachim Frank
Feb 21, 2009·The EMBO Journal·Jan-Christian SchuetteChristian M T Spahn
Mar 8, 2011·The EMBO Journal·Xabier AgirrezabalaJoachim Frank
Jul 21, 2012·Molecular Microbiology·Usheer KanjeeWalid A Houry
Jan 27, 2015·Journal of Structural and Functional Genomics·Tatsuo YanagisawaShigeyuki Yokoyama
Oct 4, 2017·Nucleic Acids Research·Karolina MichalskaChristopher S Hayes
Jul 24, 2018·Proteins·Huan YangPaul C Whitford
Oct 7, 1998·Protein Science : a Publication of the Protein Society·A RajpalJ F Kirsch
Jan 2, 1997·Nature·R A Cross
Sep 24, 1999·Biological Chemistry·J MüllerE Mandelkow
Jul 13, 2000·Protein Science : a Publication of the Protein Society·M AltmanS Zhang

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