An alternate method for extracting DNA from environmentally challenged teeth for improved DNA analysis

Legal Medicine
Sheree Hughes-StammAngela van Daal

Abstract

A grinding-free method to extract DNA from teeth via a direct minimal-invasive retrograde approach to the pulp cavity and dentine was compared to a standard grinding/pulverisation method. This alternate method uses endodontic dental files to access the root canals and pulp cavity for tissue and dentine harvest via the apical end of the roots and avoids mechanical damage to the crown and root morphology. In contrast, other methods require pulverisation of the whole root or tooth, transection or destruction of the occlusal surface to gain access to the DNA in the root canals and pulp chamber. This study compared two methods for preparing dentine powder from the roots of environmentally challenged teeth for forensic DNA analysis. We found that although the filing method was more laborious, and produced less dentine powder, the amount of amplifiable DNA per milligram of powder was substantially higher with the filing method compared to grinding the entire root. In addition, the number of short tandem repeat (STR) alleles detected and the peak height ratios of the STR profiles were notably higher. Although several other methods of extracting DNA-rich tissue from the pulp chamber of teeth have previously been reported, the method pre...Continue Reading

References

Jan 1, 1996·International Journal of Legal Medicine·A Alvarez GarcíaA Carracedo
Jun 1, 2002·The American Journal of Forensic Medicine and Pathology·R TrivediV K Kashyap
Aug 20, 2004·Journal of Forensic Sciences·Calvin Y ShiromaKenneth N Dunn
Nov 16, 2006·International Journal of Legal Medicine·Myung Jin ParkKyoung-Jin Shin
Jul 21, 2007·Nature Protocols·Nadin Rohland, Michael Hofreiter
Oct 22, 2009·American Journal of Physical Anthropology·Seung Bum SeoSoong Deok Lee
Apr 27, 2010·International Journal of Legal Medicine·Sheree R Hughes-StammAngela van Daal
Jul 29, 2010·Forensic Science International : Synergy·Françoise TilottaPhilippe de Mazancourt
Nov 11, 2010·The American Journal of Forensic Medicine and Pathology·Cecilia SosaBegoña Martínez-Jarreta
Oct 25, 2011·Journal of Forensic Dental Sciences·J Muruganandhan, G Sivakumar

❮ Previous
Next ❯

Citations

Apr 2, 2020·Molecular Genetics & Genomic Medicine·Ba Hoang Anh MaiGérard Aboudharam
Aug 4, 2021·Forensic Science International : Synergy·Rabiah A RahmatJeremy J Austin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Forensic Science International : Synergy
Françoise TilottaPhilippe de Mazancourt
Hellenika stomatologika chronika. Hellenic stomatological annals
F A Mantis
© 2021 Meta ULC. All rights reserved