PMID: 11929604Apr 4, 2002Paper

An alternate targeting pathway for procathepsin L in mouse fibroblasts

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Kyujeong AhnAnn Erickson

Abstract

In transformed mouse fibroblasts, a significant proportion of the lysosomal cysteine protease cathepsin L remains in cells as an inactive precursor which associates with membranes by a mannose phosphate-independent interaction. When microsomes prepared from these cells were resolved on sucrose gradients, this procathepsin L was localized in dense vesicles distinct from those enriched for growth hormone, which is secreted constitutively when expressed in fibroblasts. Ultrastructural studies using antibodies directed against the propeptide to avoid detection of the mature enzyme in lysosomes revealed that the proenzyme was concentrated in dense cores within small vesicles and multivesicular endosomes which labeled with antibodies specific for CD63. Consistent with the resemblance of these cores to those of regulated secretory granules, secretion of procathepsin L from fibroblasts was modestly stimulated by phorbol, 12-myristate, 13-acetate. When protein synthesis was blocked with cycloheximide and lysosomal proteolysis inhibited with leupeptin, procathepsin L was found to gradually convert to the active single-chain protease. The data suggest that when synthesis levels are high, a portion of the procathepsin L is packaged in dens...Continue Reading

References

Aug 1, 1991·Current Opinion in Cell Biology·K von Figura
Dec 1, 1991·Cell Biology International Reports·L A Ginsel, J A Fransen
Mar 1, 1991·The Journal of Cell Biology·L Matsuuchi, R B Kelly
Nov 1, 1989·The Journal of Cell Biology·M P LisantiE Rodriguez-Boulan
Jan 1, 1989·Annual Review of Cell Biology·S Kornfeld, I Mellman
Mar 15, 1989·International Journal of Cancer. Journal International Du Cancer·R A MaciewiczC Paraskeva
Oct 16, 1989·Biochemical and Biophysical Research Communications·J RozhinB F Sloane
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B F SloaneK V Honn
Nov 15, 1985·European Journal of Biochemistry·F MainfermeK von Figura
Oct 1, 1984·Cell Biology International Reports·S Craig, C Miller
Jul 1, 1983·Japanese Circulation Journal·S DokoH B Wheeler
Nov 10, 1995·The Journal of Biological Chemistry·R PohlmannK von Figura
Jun 30, 1995·The Journal of Biological Chemistry·F AuthierJ J Bergeron
Apr 1, 1994·The Histochemical Journal·K HamamotoK Yasunaga
Oct 1, 1993·The Journal of Cell Biology·J N Glickman, S Kornfeld
May 10, 1996·The Journal of Biological Chemistry·M A BittnerR W Holz
Jun 1, 1996·The Journal of Cell Biology·R A ChavezH P Moore
Jan 27, 1997·International Journal of Cancer. Journal International Du Cancer·C IsidoroG Bonelli
Aug 1, 1997·Current Biology : CB·C ThieleW B Huttner
Apr 30, 1998·Advances in Experimental Medicine and Biology·G D GodboldA H Erickson
Nov 14, 1998·Biochemical and Biophysical Research Communications·K IshidohE Kominami
Dec 23, 1998·Trends in Cell Biology·W Stoorvogel
Mar 10, 2000·Biochimica Et Biophysica Acta·J MaiB F Sloane
May 9, 2000·The Journal of Biological Chemistry·M D Turner, P Arvan
Apr 18, 2001·Trends in Cell Biology·S A ToozeW B Huttner
Nov 1, 1992·Trends in Cell Biology·P Arvan, D Castle

❮ Previous
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Citations

Jan 28, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Aikaterini VaranouMyriam Hemberger
Sep 27, 2003·European Journal of Biochemistry·Jeffrey P BocockAnn H Erickson
Oct 7, 2010·The Journal of Biological Chemistry·Janis NoonanVeronica A Campbell
May 3, 2008·The Journal of Biological Chemistry·Ghada Abboud-JarrousIsrael Vlodavsky
Jul 16, 2005·Traffic Injury Prevention·Paul C IvancicS Elena Gimenez
Aug 3, 2007·The Journal of Clinical Investigation·Sanja SeverJochen Reiser
Mar 13, 2013·The Journal of Clinical Investigation·Benjamin J CapocciaJason C Mills
Jul 7, 2005·Traffic·David G RobinsonGiselbert Hinz
Sep 8, 2004·International Journal of Cancer. Journal International Du Cancer·John ColletteAnn H Erickson
Nov 13, 2007·Biology of the Cell·Susannah D BrydgesVern B Carruthers
Dec 17, 2009·Experimental Dermatology·Anke KloseNicolas Hunzelmann
Aug 9, 2005·Biochimica Et Biophysica Acta·Alfonso González-NoriegaJorge Antonio Sosa Melgarejo
Jun 6, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Victoria Del Gaizo, R Mark Payne
Jun 5, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Jian LiuGuo-Ping Shi
Jun 20, 2002·The Journal of Biological Chemistry·Jennifer E KoblinskiBonnie F Sloane

❮ Previous
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