An alternative approach in the structure-based predictions of the thermodynamics of protein unfolding

Biophysical Chemistry
Danilo MilardiD Grasso

Abstract

A new approach for a first-order prediction of the thermodynamic properties of small globular proteins has been developed. The method put forward here has been shown to be successful in predicting, within acceptable margins of uncertainty, the denaturational heat capacity changes of a given protein if its amino acid composition is known. If compared with other models this method has the following advantages: (1) no details about the three-dimensional structure of the protein are required; (2) comparison with the thermodynamic properties of small model compounds is not necessary; (3) the temperature dependence of the denaturational heat capacity change is taken into account. Moreover, the equations developed have allowed us to point out the errors that can be made if the temperature-dependence of the denaturational heat capacity change is not taken into account in the calculation of the unfolding thermodynamic functions.

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J M Sturtevant
Dec 5, 1991·Journal of Molecular Biology·K P Murphy, S J Gill
Feb 20, 1989·Journal of Molecular Biology·P L PrivalovN N Khechinashvili
Jul 5, 1974·Journal of Molecular Biology·P L Privalov, N N Khechinashvili

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Citations

Feb 6, 2003·International Journal of Biological Macromolecules·R GuzziL Sportelli
Sep 27, 2000·Biochimica Et Biophysica Acta·M HäckelH Hinz
Apr 19, 2000·The Biochemical Journal·S TaudteN R Kallenbach
Jun 15, 2011·Journal of Pharmaceutical Sciences·Rebecca K BrummittChristopher J Roberts
Aug 8, 2008·Journal of Pharmaceutical Sciences·William F WeissChristopher J Roberts
Nov 28, 2009·Journal of Molecular Biology·Alejandra Hernández-SantoyoAdela Rodríguez-Romero
Oct 11, 2013·The FEBS Journal·Martín González-AndradeLuis del Pozo-Yauner
Jul 25, 2015·Physical Chemistry Chemical Physics : PCCP·Sergio Romero-RomeroD Alejandro Fernández-Velasco
Sep 18, 2007·Chembiochem : a European Journal of Chemical Biology·Gaetano D ManettoCarmelo La Rosa
Jul 30, 2020·Protein Engineering, Design & Selection : PEDS·Magdalena WójcikYkelien L Boersma
Sep 13, 2017·The FEBS Journal·Oscar D Luna-MartínezBaltazar Becerril
Dec 17, 2014·The Journal of Biological Chemistry·Miryam I VillalbaBaltazar Becerril
Aug 19, 2008·Biochimica Et Biophysica Acta·Rita GuzziChristopher Dennison

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