An alternative in vivo system for testing angiogenic potential of human neuroblastoma cells

Cancer Letters
Domenica MangieriDomenico Ribatti

Abstract

In this study we purposed an alternative method to study the angiogenic and invasive potential of neuroblastoma cell suspensions implanted on the chick embryo chorioallantoic membrane (CAM) surface. Neuroblastoma cells were seeded in Matrigel and thereafter the suspension was pipetted onto the CAM surface at day 8 of incubation inside a silicon ring previously loaded onto the CAM surface. Four days after implantation, the silicon ring was removed and the angiogenic and invasive response were studied morphologically at macroscopic and microscopic levels and by reverse transcriptase-polymerase chain reaction (RT-PCR) by using human and chicken primers for several angiogenic cytokines, namely vascular endothelial growth factor-A (VEGF-A), fibroblast growth factor-2 (FGF-2), angiopoietin-1 (ANG-1), hypoxia inducible factor-2alpha (HIF-2alpha), and for an endogenous angiostatic molecule, namely endostatin. Results showed that: (1) Neuroblastoma cells induced an angiogenic response in the CAM assay comparable to that induced by FGF-2; (2) neuroblastoma cells are packed inside Matrigel or are recognizable in the CAM mesenchyme; (3) Angiogenic activity of neuroblastoma cells is associated to an high expression of the transcripts of hum...Continue Reading

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Citations

Nov 10, 2009·Journal of Experimental & Clinical Cancer Research : CR·Gordana JakovljevićSven Seiwerth
Jan 29, 2011·International Endodontic Journal·L-L WeiP Buxton
Aug 17, 2011·Journal of Experimental & Clinical Cancer Research : CR·Jun WanYun Che
May 27, 2015·Journal of Cellular and Molecular Medicine·Carlotta AliasDomenica Mangieri
Feb 15, 2018·International Journal of Molecular Sciences·Katarzyna Zabielska-KoczywąsRoman Lechowski
Aug 6, 2018·Journal of Cellular Physiology·Domenico Ribatti, Roberto Tamma
Aug 11, 2011·The Journal of Pharmacology and Experimental Therapeutics·Yuanyuan WuXiufeng Pang
Dec 30, 2015·PeerJ·Young Bin KimChang Hun Kim

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