An alternative model of H ferritin promoter transactivation by c-Jun

The Biochemical Journal
M C FanielloF Costanzo

Abstract

c-Jun is a member of the activator protein 1 family, and its interaction with different nuclear factors generates a wide spectrum of complexes that regulate transcription of different promoters. H ferritin promoter transcription is tightly dependent on nuclear factor Y (NFY). Ferritin transcription is activated by c-Jun, although the promoter does not contain a canonical binding site. NFY, on the other hand, does not bind c-Jun in vitro, whereas in vivo c-Jun is found in the complex containing NFY. Moreover, a c-Jun-GCN4 chimaeric construct containing only the transactivation domain of Jun and the basic-region leucine-zipper domain of GCN4 stimulates the H ferritin promoter. A synthetic GAL4 promoter and the cognate activator, the fusion protein NFY-GAL4, are potently activated by c-Jun. Titration of p300 by co-expressing E1A abolishes the stimulatory effect. Moreover, another p300-dependent promoter, the cAMP-response element, can be superactivated by c-Jun using the same mechanism. These data indicate that c-Jun, when activated or overexpressed, is recruited to the H ferritin promoter by p300, which links NFY, bound to DNA, to the complex. These results add a new level of complexity to transcriptional regulation by c-Jun, whi...Continue Reading

References

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Feb 13, 1999·The Journal of Biological Chemistry·G BehreD G Tenen
Mar 13, 1999·The Journal of Biological Chemistry·M C FanielloF Costanzo

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Citations

Apr 6, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Anna Cook Abt, Olimpia Meucci
Feb 18, 2016·Experimental Eye Research·James K KubilusThomas F Linsenmayer
Mar 29, 2008·The International Journal of Biochemistry & Cell Biology·Maria Concetta FanielloFrancesco Costanzo
Apr 2, 2004·Cellular Microbiology·Robert A BonnahMagdalene So
Nov 5, 2011·Critical Reviews in Biochemistry and Molecular Biology·Diletta DolfiniRoberto Mantovani
Mar 31, 2015·PloS One·Flavia BiamonteFrancesco Costanzo

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