An alternative non-tyrosine protein kinase product of the c-src gene in chicken skeletal muscle.

Molecular and Cellular Biology
T Dorai, L H Wang

Abstract

While the c-src locus is expressed as a 4.0-kilobase (kb) mRNA coding for pp60c-src in various chicken tissues, including embryonic muscle, it is expressed as a novel 3.0-kb mRNA in adult skeletal muscle. We have analyzed the primary structure of this alternatively transcribed and spliced c-src mRNA. The sequence revealed three open reading frames, with the previously defined c-src exons 1 through 5 or 6 comprising the third, on the 3' untranslated region of this 3-kb mRNA. The exons coding for the tyrosine kinase domain of pp60c-src were excluded. On the 5' side, 2 kb of sequence upstream from the previously defined exon 1 of the c-src gene was included in this mRNA. The start site for the 3-kb mRNA probably lies downstream of that for the 4-kb mRNA. The first reading frame of the 3.0-kb mRNA, called sur (for src upstream region), encoded a 24-kilodalton (kDa) protein product rich in cysteine and proline residues. In vitro analysis indicated that the 24-kDa sur protein was membrane associated. Antibodies to sur protein detected in vivo a 24-kDa muscle-specific protein which was developmentally regulated and corresponded to the switch from the 4-kb to the 3-kb c-src mRNA. A striking kinetic pattern of appearance of sur protein ...Continue Reading

Citations

Aug 7, 2007·Cytogenetic and Genome Research·S KatyalR Godbout
Dec 14, 1999·The Journal of Biological Chemistry·P YangS Marcus
Sep 15, 1995·The Journal of Biological Chemistry·P I DuncanJ C Bell
Dec 1, 1992·Journal of Neurochemistry·B GrimaB Pessac
Feb 1, 1992·The International Journal of Biochemistry·J E Hesketh, P F Whitelaw

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