An alternative pathway for expression of p56lck from type I promoter transcripts in colon carcinoma

Oncogene
S McCrackenN G Miyamoto

Abstract

The lymphoid-specific protein tyrosine kinase, p56lck which is essential for both T cell development and function, is aberrantly expressed in colon and small lung carcinoma lines. In this paper, we demonstrate p56lck is also expressed in colon tumour biopsies due predominantly or exclusively to the use of the lck type I promoter. In T leukaemia lines, the lck type I promoter requires binding sites for both Ets- and Myb-related transcription factors. In contrast, in colon tumour lines the activation of the lck type I promoter requires the Ets but not the Myb binding site. In these lines, a consensus binding site for HMG-related transcription factors, AACAAAG, is required for efficient lck type I promoter activity. Sox-4 is a candidate transcription factor for binding and activating the lck type l promoter in colon carcinoma cells. Co-expression of Ets-1 and Sox-4, but neither protein alone, was sufficient to activate the lck type l promoter in HeLa cells which do not normally express lck transcripts. These results suggest that aberrant expression of p56lck from the lck type l promoter in colon carcinoma arises from transcriptional activation mediated by Ets- and HMG-related transcription factors.

Citations

Apr 4, 2003·Critical Reviews in Oncology/hematology·Piero DalerbaGiorgio Parmiani
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Carl VirtanenMichael H Jones
Apr 25, 2000·Drugs·H H Sedlacek
Nov 4, 2004·Biochemical and Biophysical Research Communications·Eun-Hye HurHyangshuk Rhim
Oct 20, 2012·Cellular and Molecular Life Sciences : CMLS·Seyed Mehdi JafarnejadGang Li
Apr 26, 2006·Oncogene·P PramoonjagoC A Moskaluk
Aug 12, 2005·International Journal of Cancer. Journal International Du Cancer·Calley L HirschKeith Bonham
Apr 25, 2006·Biochemical and Biophysical Research Communications·Xin PanXuemin Zhang
Nov 16, 2012·Blood·Parham Ramezani-RadMarkus Müschen
Nov 21, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nadine KosslerArno Kalkuhl
May 19, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Bangly SolimanMahmoud El Hefnawi
Jan 1, 2008·Cancer Informatics·Gino BoilyDaniel Sinnett

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