An alternative RNA polymerase I structure reveals a dimer hinge

Acta Crystallographica. Section D, Biological Crystallography
Dirk KostrewaPatrick Cramer

Abstract

RNA polymerase I (Pol I) is the central, 14-subunit enzyme that synthesizes the ribosomal RNA (rRNA) precursor in eukaryotic cells. The recent crystal structure of Pol I at 2.8 Å resolution revealed two novel elements: the `expander' in the active-centre cleft and the `connector' that mediates Pol I dimerization [Engel et al. (2013), Nature (London), 502, 650-655]. Here, a Pol I structure in an alternative crystal form that was solved by molecular replacement using the original atomic Pol I structure is reported. The resulting alternative structure lacks the expander but still shows an expanded active-centre cleft. The neighbouring Pol I monomers form a homodimer with a relative orientation distinct from that observed previously, establishing the connector as a hinge between Pol I monomers.

References

Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·R J Read
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Katherine A Kantardjieff, Bernhard Rupp
Dec 28, 2007·Cell·Claus-D KuhnPatrick Cramer
Jan 9, 2010·Annual Review of Pharmacology and Toxicology·Denis DryginIngrid Grummt
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 9, 2010·Nature·Gunnar F SchröderAxel T Brunger
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Aug 28, 2010·Molecular Cell·Sebastian R GeigerPatrick Cramer
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Sep 24, 2011·Genes & Development·Claudia BlattnerPatrick Cramer
Mar 8, 2012·Nucleic Acids Research·Stefan JennebachPatrick Cramer
Aug 21, 2013·Trends in Molecular Medicine·Nadine HeinRoss D Hannan
Oct 25, 2013·Nature·Christoph EngelPatrick Cramer
Oct 25, 2013·Nature·Carlos Fernández-TorneroChristoph W Müller

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Citations

Jul 16, 2016·Nature Communications·Michael PilslPatrick Schultz
Jul 16, 2016·Nature Communications·Christoph EngelPatrick Cramer
May 25, 2018·Annual Review of Biophysics·Christoph EngelPatrick Cramer
Aug 17, 2016·Biochemical Society Transactions·Patrick Cramer
Feb 16, 2020·The Journal of Biological Chemistry·Philipp E MerklHerbert Tschochner
Mar 7, 2020·Nature Communications·Michael Pilsl, Christoph Engel
Nov 15, 2016·Nature·Simon NeyerAchilleas S Frangakis
Feb 5, 2021·Nature Communications·Florian B HeissChristoph Engel
Sep 17, 2021·Nucleic Acids Research·Shintaro AibaraPatrick Cramer

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