An amino acid substitution in PBP-3 in Haemophilus influenzae associate with the invasion to bronchial epithelial cells

Microbiological Research
Tadashi OkabeHideji Yanagisawa

Abstract

Haemophilus influenzae is a common pathogen of respiratory infections. We examined whether beta-lactamase-negative ampicillin-resistant (BLNAR) strains that are known to have ampicillin resistance due to a substitution of amino acid of penicillin binding protein (PBP)-3, differ from beta-lactamase-negative ampicillin-susceptible strains with regard to invasion of bronchial epithelium. After 3h incubation of each of 34 beta-lactamase-negative ampicillin-susceptible and 57 BLNAR strains in the presence of BEAS-2B cells, a human bronchial epithelium cell line, extracellular bacteria were killed using gentamicin and intracellular bacteria numbered. All nine strains in which the efficiency of invasion was 1% or higher were BLNAR strains. The rate of invasion was significantly greater in strains with PBP-3 amino acid substitution (Met377 to Ile, Ser385 to Thr, Leu389 to Phe, and Asn526 to Lys) (n=34) than in those with no amino acid substitution. Electron microscopy showed that high invasive BLNAR strains were observed in cytoplasm of BEAS-2B cell layer. The injured cells were 9.44+/-1.76% among attaching cells examined by trypan blue staining after 6h. These data may suggest that the amino acid substitution of the PBP in BLNAR strai...Continue Reading

References

Dec 1, 1978·The Journal of Infectious Diseases·A R Flesher, R A Insel
Apr 1, 1990·The Journal of Antimicrobial Chemotherapy·P M MendelmanG Kalaitzoglou
Feb 10, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·W A Craig
Sep 15, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J G BartlettM J Fine
Oct 13, 2000·American Journal of Physiology. Cell Physiology·M Marinò, R T McCluskey
Jan 26, 2002·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·H KunishimaJ Shimada
Apr 3, 2002·JAMA : the Journal of the American Medical Association·Garth D EhrlichJ Christopher Post
Aug 16, 2002·The New England Journal of Medicine·Sanjay SethiTimothy F Murphy
Jul 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Valérie BouchetE Richard Moxon
May 27, 2005·Antimicrobial Agents and Chemotherapy·Martina BosnarMichael J Parnham
Jan 5, 2006·Applied and Environmental Microbiology·I GantoisF Van Immerseel
Mar 7, 2006·Thorax·E Sapey, R A Stockley
Apr 29, 2006·Current Opinion in Infectious Diseases·Timothy F Murphy
Sep 1, 2007·Journal of Cellular and Molecular Medicine·Arwyn Tomos Jones

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Citations

Jun 13, 2012·Antimicrobial Agents and Chemotherapy·Fredrik ResmanKristian Riesbeck
Jan 5, 2010·The Tohoku Journal of Experimental Medicine·Yoichi HirakataMitsuo Kaku
Sep 27, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Chong LuoYu Shi
Feb 27, 2015·Proceedings. Biological Sciences·Alejandro CouceJesús Blázquez
Jul 31, 2016·Journal of Microbiological Methods·Neeraj Kumar SinghStephen G Tristram
Sep 15, 2016·Journal of Medical Microbiology·Neeraj Kumar SinghStephen G Tristram

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