An amino-terminal fragment of urokinase isolated from a prostate cancer cell line (PC-3) is mitogenic for osteoblast-like cells

Biochemical and Biophysical Research Communications
S A RabbaniD Goltzman

Abstract

A peptide mitogen for cultured osteoblast-like cells was purified from serum-free conditioned culture medium of a human prostatic cancer cell line, PC-3. Based on amino acid sequencing and estimated molecular weight, this peptide was identified as an NH2-terminal fragment of urokinase-type plasminogen activator (uPA). Recombinant high molecular weight (HMW) uPA and the NH2-terminal growth factor domain (GFD) of uPA, but not low molecular weight (LMW) uPA (lacking the NH2-terminal region) stimulated [3H] thymidine incorporation and proliferation in osteoblast-like cells, and specific, competitive binding sites for HMW, but not LMW, uPA were demonstrable. These studies demonstrate the production of a mitogenic NH2-terminal fragment of uPA by a human prostatic cancer cell line which may be of importance in the pathogenesis of osteoblastic metastases.

References

Aug 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J C KirchheimerB R Binder
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Oct 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W A GünzlerL Flohé
Nov 1, 1980·Urology·S C Jacobs, R K Lawson

❮ Previous
Next ❯

Citations

Jul 29, 1996·International Journal of Cancer. Journal International Du Cancer·R H Xing, S A Rabbani
Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·P A AndreasenM J Duffy
Apr 1, 1996·Journal of Cellular Biochemistry·J MartínezJ F Santibáñez
Jun 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G LeloupG Vaes
Nov 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M KoutsilierisC J Doillon
Sep 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A C de BartJ H Verheijen
Aug 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G LeloupG Vaes
Nov 1, 1992·Cancer Metastasis Reviews·H C Kwaan
Apr 27, 2007·Current Urology Reports·Bulent Akduman, E David Crawford
Aug 10, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Anjali MishraRussell S Taichman
Jan 1, 1995·Critical Reviews in Oncology/hematology·M Koutsilieris
Oct 25, 2003·Progress in Retinal and Eye Research·Arup Das, Paul G McGuire
Jan 5, 2005·Nature Reviews. Cancer·Christopher J Logothetis, Sue-Hwa Lin
May 11, 2011·Nature Reviews. Clinical Oncology·Justin SturgeJonathan Waxman
Feb 3, 2005·Cell Research·Juan Juan YinKathleen Kelly
Jan 19, 2002·Differentiation; Research in Biological Diversity·F ElfmanG Cunha
Jul 17, 1999·European Journal of Biochemistry·J A Aguirre GhisoE B de Kier Joffè
Oct 9, 2001·BJU International·F C Hamdy, B G Thomas
Apr 16, 2004·The New England Journal of Medicine·G David Roodman
Aug 1, 1993·The Journal of Cell Biology·M S PepperJ D Vassalli
Nov 1, 1993·The Journal of Clinical Investigation·N PedersenF Jänicke
May 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J N YangC Minkin
Apr 26, 2011·Progrès en urologie : journal de l'Association française d'urologie et de la Société française d'urologie·T LebretN Houédé
Apr 17, 2009·Canadian Journal of Physiology and Pharmacology·Vsevolod A TkachukYelena V Parfyonova
Sep 13, 2013·Expert Opinion on Investigational Drugs·Pavlos MsaouelMichael Koutsilieris
Jul 18, 2008·Cancer Letters·Jeroen T Buijs, Gabri van der Pluijm
Jan 29, 2010·Cancer·Toni IbrahimDino Amadori
Apr 14, 2016·Cancer Metastasis Reviews·Kosei NakajimaAvraham Raz
Jul 5, 2005·Expert Opinion on Investigational Drugs·U H Weidle, B König
Jan 5, 2002·Expert Opinion on Investigational Drugs·C S Mitsiades, M Koutsilieris
Jan 1, 1993·Scandinavian Journal of Urology and Nephrology·N W ClarkeN J George
Apr 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C Luparello, M Del Rosso
Jan 14, 1999·The Journal of Investigative Dermatology·T HibinoP F Goetinck

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.