An amperometric chloramphenicol immunosensor based on cadmium sulfide nanoparticles modified-dendrimer bonded conducting polymer

Biosensors & Bioelectronics
Dong-Min KimYoon-Bo Shim

Abstract

An amperometric chloramphenicol (CAP) immunosensor was fabricated by covalently immobilizing anti-chloramphenicol acetyl transferase (anti-CAT) antibody on cadmium sulfide nanoparticles (CdS) modified-dendrimer that was bonded to the conducting polymer (poly 5, 2': 5', 2''-terthiophene-3'-carboxyl acid (poly-TTCA)) layer. The AuNPs, dendrimers, and CdS nanoparticles were deposited onto the polymer layer in order to enhance the sensitivity of the sensor probes. The particle sizes were determined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The immobilization of dendrimers, CdS, and anti-CAT were confirmed using energy disruptive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), and quartz crystal microbalance (QCM) techniques. The detection of CAP was based on the competitive immuno-interaction between the free- and labeled-CAP for active sites of the anti-CAT. Hydrazine was used as the label for CAP, and it electrochemically catalyzed the reduction of H(2)O(2) at -0.35 V vs. Ag/AgCl. Under optimized conditions, the proposed immunosensor exhibited a linear range of CAP detection between 50 pg/mL and 950 pg/mL, and the detection limit was 45 pg/mL. The immunosensor was examined in...Continue Reading

References

Aug 1, 1976·Antimicrobial Agents and Chemotherapy·P S LietmanW V Shaw
Mar 30, 2002·Science·Wendy U HuynhA Paul Alivisatos
Oct 31, 1947·Science·J EhrlichP R Burkholder

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Citations

Jan 1, 2010·Sensors·Anees A AnsariAbdullah S Aldwayyan
Aug 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana M GonçalvesLuís A Passarinha
Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Phuong Dinh Tam, Cao Xuan Thang
Jul 21, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·Xiao Feng YangHong Qun Luo
May 3, 2011·Analytical Biochemistry·Kyung-Mi SongChangill Ban
Apr 2, 2015·Critical Reviews in Food Science and Nutrition·Sanaz PilehvarKarolien De Wael
Jan 13, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Mona AlibolandiMohammad Ramezani
Jul 5, 2017·Nanoscale·Ioulia TzouvadakiSandro Carrara
Jan 10, 2012·Analytical and Bioanalytical Chemistry·Kyung-Mi SongChangill Ban
Mar 8, 2020·Journal of the Science of Food and Agriculture·Mustafa Oguzhan Caglayan
Aug 18, 2012·Journal of Molecular Recognition : JMR·Robert E Speight, Matthew A Cooper
Dec 11, 2019·Nanomaterials·Alfredo SánchezReynaldo Villalonga

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