An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

Journal of Neuroscience Methods
John P LowryMarianne Fillenz

Abstract

Amperometric glucose biosensors based on the immobilization of glucose oxidase (GOx) on Pt electrodes with electropolymerized o-phenylenediamine (PPD) were implanted in the right striatum of freely-moving rats. Carbon paste electrodes for the simultaneous monitoring of ascorbic acid (AA) and/or tissue O2 were implanted in the left striatum. A detailed in vivo characterization of the Pt/PPD/GOx signal was carried out using various pharmacological manipulations. Confirmation that the biosensor responded to changing glucose levels in brain extracellular fluid (ECF) was obtained by intraperitoneal (i.p.) injection of insulin that caused a decrease in the Pt/PPD/GOx current, and local administration of glucose (1 mM) via an adjacent microdialysis probe that resulted in an increase in the biosensor current. An insulin induced increase in tissue O2 in the brain was also observed. Interference studies involved administering AA and subanaesthetic doses of ketamine i.p. Both resulted in increased extracellular AA levels with ketamine also causing an increase in O2. No significant change in the Pt/PPD/GOx current was observed in either case indicating that changes in O2 and AA, the principal endogenous interferents, have minimal effect on...Continue Reading

References

Jan 1, 1991·Analytical Chemistry·J B Zimmerman, R M Wightman
Jan 1, 1990·Biosensors & Bioelectronics·G FortierD Bélanger
Dec 23, 1986·Neuroscience Letters·M G BoutelleP N Bartlett
Dec 1, 1980·Journal of Neuroscience Methods·G PaxinosP C Emson
Apr 1, 1984·Analytical Chemistry·A E CassA P Turner
Apr 1, 1993·Journal of Pharmacological and Toxicological Methods·Z Chen, R W Steger
Aug 1, 1996·Analytical Chemistry·M N FriedemannG A Gerhardt
Dec 1, 1996·Journal of Neuroscience Methods·M Miele, M Fillenz

❮ Previous
Next ❯

Citations

Jan 29, 2013·Applied Biochemistry and Biotechnology·Anish KhanSulaiman Ab Ghani
Sep 18, 2002·Journal of Neuroscience Methods·Shawn F DressmanAdrian C Michael
Feb 11, 2014·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Marta TibilettiJeremy C T Fairbank
Dec 10, 2014·ACS Chemical Neuroscience·Eugene A Kiyatkin, Ken T Wakabayashi
Feb 18, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sarah E HopwoodAnthony J Strong
Apr 28, 2005·Biosensors & Bioelectronics·George S Wilson, Raeann Gifford
Dec 14, 2004·Journal of Neuroscience Methods·Paul A GarrisGeorge V Rebec
Jun 21, 2005·Neuroscience and Biobehavioral Reviews·Marianne Fillenz
Jun 14, 2005·Trends in Biotechnology·Nicholas DaleEnrique Llaudet
Dec 1, 2010·Journal of Neuroscience Methods·Fiachra B BolgerJohn P Lowry
Feb 13, 2001·Methods : a Companion to Methods in Enzymology·T P Obrenovitch, E Zilkha
May 16, 2015·Biosensors & Bioelectronics·Natalia VasylievaAndrei Sabac
Dec 9, 2016·Journal of Neurophysiology·Adam J H NewtonMagnus J E Richardson
Dec 13, 2006·The European Journal of Neuroscience·John P BrunoGreg A Gerhardt
Feb 15, 2018·Journal of Neuroengineering and Rehabilitation·Scott D AdamsMichael Berk
Sep 12, 2002·Chemical Reviews·Xin-Gui LiYu-Liang Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.