An amperometric immunosensor based on a polyelectrolyte/gold magnetic nanoparticle supramolecular assembly--modified electrode for the determination of HIV p24 in serum.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Ning GanYuting Cao

Abstract

A novel supramolecular amperometric immunosensor for the determination of Human Immunodeficiency Virus antigen p24 (HIV p24) was built up using the electrostatic layer-by-layer self-assembly technique upon a gold electrode with HIV p24 antibody (anti-p24) being immobilized on polyelectrolyte/gold nanoparticle multilayer films. The multilayer films were composed of poly(L-lysine) (pLys) and mercaptosuccinic acid (MSA) stabilized Fe(3)O(4)(core)/gold(shell) nano particles (GMPs).The immunosensor preparation steps were monitored by X-ray fluorescence spectrometry (XRFS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In pH 6.5 PBS, after the immunosensor was incubated with HIV p24 solution at 25 degrees C for 5 min, the electron transfer access of FeCN is partially inhibited, which leads to a linear decrease of peak current. In addition, the performance of the immunosensor was studied in detail. It offers high-sensitivity for the detection of p24 and has good correlation for the detection of p24 in the range of 0.1 to 100.0 ng/mL with a detection limit of 0.05 ng/mL estimated at a signal-to-noise ratio of 3. The proposed immunosensor was used to analyze p24 in human serum specimens and the results s...Continue Reading

References

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Citations

Jan 10, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyunje WooKang Hyun Park
Aug 31, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Tejabhiram Yadavalli, Deepak Shukla
Sep 11, 2014·ACS Applied Materials & Interfaces·Zhaohao WangNianjun Yang

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Methods Mentioned

BETA
enzyme-linked immunoassay assay
biosensors
biosensor
ELISA
scanning electron microscopy
transmission electron microscopy
X-ray

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