An amperometric penicillin biosensor with enhanced sensitivity based on co-immobilization of carbon nanotubes, hematein, and beta-lactamase on glassy carbon electrode

Analytica Chimica Acta
Bi ChenXiaoli Su

Abstract

An amperometric penicillin biosensor with enhanced sensitivity was successfully developed by co-immobilization of multi-walled carbon nanotubes (MWCNTs), hematein, and beta-lactamase on glassy carbon electrode using a layer-by-layer assembly technique. Under catalysis of the immobilized enzyme, penicillin was hydrolyzed, decreasing the local pH. The pH change was monitored amperometrically with hematein as a pH-sensitive redox probe. MWCNTs were used as an electron transfer enhancer as well as an efficient immobilization matrix for the sensitivity enhancement. The effects of immobilization procedure, working potential, enzyme quantity, buffer concentration, and sample matrix were investigated. The biosensor offered a minimum detection limit of 50 nM (19 microg L(-1)) for penicillin V, lower than those of the conventional pH change-based biosensors by more than two orders of magnitude. The electrode-to-electrode variation of the response sensitivity was 7.0% RSD.

Citations

Jan 23, 2013·Analytical and Bioanalytical Chemistry·Terry F McGrathChristopher T Elliott
Jul 26, 2014·International Journal of Nanomedicine·Congo Tak-Shing ChingHsiu-Li Shieh
Feb 6, 2015·Biomedical Microdevices·Mohammed M Rahman, Abdullah M Asiri
Aug 9, 2020·Journal of Biological Engineering·Nina LautenschlägerThorsten Mascher
Jan 27, 2012·Analytical and Bioanalytical Chemistry·T F McGrathT L Fodey
Jul 23, 2020·Biotechnology Letters·Tannu KharewalVikas Hooda

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