An Amplicon-Based Approach for the Whole-Genome Sequencing of Human Metapneumovirus.

Rachel L TullochJohn-Sebastian Eden


Human metapneumovirus (HMPV) is an important cause of upper and lower respiratory tract disease in individuals of all ages. It is estimated that most individuals will be infected by HMPV by the age of five years old. Despite this burden of disease, there remain caveats in our knowledge of global genetic diversity due to a lack of HMPV sequencing, particularly at the whole-genome scale. The purpose of this study was to create a simple and robust approach for HMPV whole-genome sequencing to be used for genomic epidemiological studies. To design our assay, all available HMPV full-length genome sequences were downloaded from the National Center for Biotechnology Information (NCBI) GenBank database and used to design four primer sets to amplify long, overlapping amplicons spanning the viral genome and, importantly, specific to all known HMPV subtypes. These amplicons were then pooled and sequenced on an Illumina iSeq 100 (Illumina, San Diego, CA, USA); however, the approach is suitable to other common sequencing platforms. We demonstrate the utility of this method using a representative subset of clinical samples and examine these sequences using a phylogenetic approach. Here we present an amplicon-based method for the whole-genome ...Continue Reading


May 30, 2002·Virology·Bernadette G van den HoogenRon A M Fouchier
Feb 25, 2003·The Journal of Infectious Diseases·Ann R FalseyEdward E Walsh
Nov 1, 2003·Virology·Stéphane BiacchesiUrsula J Buchholz
Jan 30, 2004·The New England Journal of Medicine·John V WilliamsJames E Crowe
Jun 15, 2004·The Journal of Infectious Diseases·Alexander J McAdamTracy A Lieu
Sep 13, 2006·Emerging Infectious Diseases·Theo P SlootsMichael D Nissen
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Jun 25, 2013·Biotechnology Letters·Li-Yeh ChuangCheng-Hong Yang
Dec 31, 2017·Influenza and Other Respiratory Viruses·Simon PollettMariana Leguia
Jun 7, 2018·The Journal of Infectious Diseases·Catherine F HoulihanEleni Nastouli
Jul 30, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Yong Kwan LimMi-Kyung Lee
Apr 17, 2020·Virus Evolution·John-Sebastian EdenUNKNOWN 2019-nCoV Study Group
Sep 11, 2020·BioRxiv : the Preprint Server for Biology·John R TysonJoshua Quick

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

© 2021 Meta ULC. All rights reserved