An amplified comparative fluorescence resonance energy transfer immunosensing of CA125 tumor marker and ovarian cancer cells using green and economic carbon dots for bio-applications in labeling, imaging and sensing

Biosensors & Bioelectronics
Somayeh Hamd-GhadarehSaman Bahrami

Abstract

CA125, is a marker in the clinical diagnosis of several cancers and currently is the best serum-based tumor marker for ovarian cancer. Here, we developed an ultrasensitive antibody-ssDNA aptamer sandwich-type fluorescence immunosensor for CA125 detection. Based on a novel signal amplification strategy the carbon dots (CDs) functionalized with aptamer (CD-aptamer) used as detection probe and PAMAM-Dendrimers/AuNPs was used for covalent attachment of CA125-antibody and completing the sandwich assay method. By measuring of fluorescence resonance energy transfer (FRET) signals between CDs and AuNPs as nanoquenchers, the fluorescence signal quenched during sandwich complex formed between anti-CA125, CA125 and CDs-Aptamer and decreasing of fluorescence response signal is related to CA125 concentrations. Under optimal conditions, the immunosensor exhibited an extremely low calculated detection limit of 0.5fg/mL with wide linear range 1.0fg/mL to 1.0ng/mL of CA 125. The application of the immunosensor for CA125 detection in serum samples and measuring of ovarian-cancer cells was also investigated. The immunosensor revealed good sensitivity and specificity with ovarian cell concentrations from 2.5×103to 2×104cells/mL with correlation co...Continue Reading

Citations

Mar 8, 2018·International Journal of Molecular Sciences·Yasar HoosenViness Pillay
Jan 6, 2018·Cancers·Shin-Ichiro HoriJiehua Zhou
Feb 20, 2019·Small·Jianjun DuXiaojun Peng
Feb 28, 2019·Annual Review of Analytical Chemistry·David T OmsteadBasar Bilgicer
Nov 25, 2017·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Hui Feng, Zhaosheng Qian
Oct 14, 2018·BMC Veterinary Research·Qi GaoZhengli Chen
Mar 10, 2019·Mikrochimica Acta·Meghdad PirsahebMehrdad Payandeh
Sep 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wujuan YanPu Wang
Sep 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wenbo ChengPeng Miao
Jul 10, 2020·Analytical and Bioanalytical Chemistry·Xinyue KouLimin Ning
Jan 10, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Minghai ZhangYu Wang
Feb 23, 2021·Macromolecular Bioscience·Yunqi GuoXiangyang Shi
Jan 29, 2020·Biosensors & Bioelectronics·Parvin Samadi PakchinYadollah Omidi
Mar 16, 2021·Frontiers in Oncology·Qurat Ul Ain ZahraZhaofeng Luo
Aug 20, 2020·ACS Sensors·Chunyu JiZhili Peng
Aug 9, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Pavel KhramtsovMikhail Rayev

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensors for Cancer Detection

Biosensors are devices that are designed to detect a specific biological analyte by essentially converting a biological entity (ie, protein, DNA, RNA) into an electrical signal that can be detected and analyzed. The use of biosensors in cancer detection and monitoring holds vast potential. Biosensors can be designed to detect emerging cancer biomarkers and to determine drug effectiveness at various target sites. Biosensor technology has the potential to provide fast and accurate detection, reliable imaging of cancer cells, and monitoring of angiogenesis and cancer metastasis, and the ability to determine the effectiveness of anticancer chemotherapy agents.