An amplified electrochemical immunosensor based on in situ-produced 1-naphthol as electroactive substance and graphene oxide and Pt nanoparticles functionalized CeO2 nanocomposites as signal enhancer

Biosensors & Bioelectronics
Zhe-Han YangYaqin Chai

Abstract

In this work, an amplified electrochemical immunosensor based on 1-naphthol as electroactive substance and Pt/CeO2/GO composites as catalytic amplifier was constructed for sensitive detection influenza. Through "sandwich" reaction, the Pt/CeO2/GO functionalized bioconjugates were captured on electrode surface and the electrochemical signal directly originated from 1-naphthol, which was in situ produced with high local concentration though the hydrolysis of 1-naphthyl phosphate catalyzed by ALP. Then, 1-naphthol as new reactant was oxidized by Pt/CeO2/GO composites with outstanding catalytic performance, resulting in detection signal amplification. In addition, as compared to label electroactive substance to antibodies, a simplified preparative step of immunosensor could be achieved because the signal probe get rid of introducation other electroactive substances. The proposed immunosensor achieved a linear range of 1.0×10(-3)-1.0ngmL(-1) and 5.0 to 1.0×10(2)ngmL(-1) with a detection limit of 0.43pgmL(-1) (defined as S/N=3).

References

Apr 27, 2007·Seminars in Respiratory and Critical Care Medicine·Joseph P Lynch, Edward E Walsh
Oct 31, 2014·Chemical Communications : Chem Comm·T T LeA E G Cass

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Citations

Aug 31, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Tejabhiram Yadavalli, Deepak Shukla
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Jul 28, 2017·Therapeutic Advances in Infectious Disease·Lavanya SinghRaveen Parboosing
Oct 21, 2018·Biosensors·Karolina DziąbowskaDawid Nidzworski
Aug 5, 2020·Biosensors & Bioelectronics·Eleni VermisoglouMichal Otyepka
Feb 9, 2021·Chemical Engineering Journal·Shariq SulemanTejraj M Aminabhavi
Nov 10, 2020·Chemical Engineering Journal·Shikandar D BukkitgarTejraj M Aminabhavi

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