PMID: 7011806Feb 1, 1981Paper

An analysis of activity determinations in a series of coupled redox reactions with special reference to hydrogenase

European Journal of Biochemistry
C van DijkC Veeger

Abstract

In this paper a spectrophotometric method is described to study the effects of pH and redox potential on the hydrogen production activity of hydrogenase, which will be applied in the following paper. The theoretical part of this paper gives the mathematical description of a system in which a redox mediator is continuously reduced by a redox system, in a non-rate-limiting way and continuously enzymatically oxidized, in a rate-limiting way. Due to the changes in redox potential during the course of the reaction the ratio reduced/oxidized redox mediator declines gradually. It is possible to relate the changes in this ratio to the actual amount of product formed. The calculation of product formation has been applied to the production of hydrogen by hydrogenase in a system with a redox mediator, whose reduced form functions as the ultimate electron donor for hydrogenase, and the dithionite/(bi)sulphite redox couple as reducing system. The changes in the ratio reduced/oxidized redox mediator as a function of pH and redox potential, followed spectrophotometrically, have been related to the hydrogen production activity of hydrogenase. The second part of the paper deals with the analysis of the theory and describes the conditions under ...Continue Reading

References

Dec 17, 1979·European Journal of Biochemistry·C Van DijkC Veeger
Dec 18, 1974·Biochimica Et Biophysica Acta·J S Chen, L E Mortenson
Jan 1, 1980·European Journal of Biochemistry·C van DijkC Veeger
Jan 1, 1956·Journal of Bacteriology·H D PECK, H GEST
Jul 20, 1933·The Journal of General Physiology·L Michaelis, E S Hill

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Citations

Mar 1, 1983·European Journal of Biochemistry·H J GrandeC Veeger
Feb 1, 1982·Analytical Biochemistry·V M FernándezA Ballesteros
Mar 28, 1986·Biochemical and Biophysical Research Communications·P Boivin, C Bourdillon

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