Nov 11, 2018

An Analysis of Characterized Plant Sesquiterpene Synthases

BioRxiv : the Preprint Server for Biology
Janani DurairajAalt Dj van Dijk

Abstract

Plants exhibit a vast array of sesquiterpenes, organic hydrocarbons which often function as herbivore-repellents or pollinator-attractants. These in turn are produced by a diverse range of sesquiterpene synthases. A comprehensive analysis of these enzymes in terms of product specificity has been hampered by the lack of a centralized resource of sufficient functionally annotated sequence data. To address this, we have gathered 262 plant sesquiterpene synthase sequences with experimentally characterized products. The annotated enzyme sequences allowed for an analysis of terpene synthase motifs, leading to the extension of one motif and recognition of a variant of another. In addition, putative terpene synthase sequences were obtained from various resources and compared with the annotated sesquiterpene synthases. This analysis indicated regions of terpene synthase sequence space which so far are unexplored experimentally. Finally, we present a case describing mutational studies on residues altering product specificity, for which we analyzed conservation in our database. This demonstrates an application of our database in choosing likely-functional residues for mutagenesis studies aimed at understanding or changing sesquiterpene sy...Continue Reading

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Mentioned in this Paper

Study
Enzymes, antithrombotic
Insect attractants
Sesquiterpenes
Terpene synthase
Enzymes for Treatment of Wounds and Ulcers
Protein Domain
Herbivory
Nitric Oxide Synthase
Mutagenesis Procedure

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