PMID: 17816Jan 1, 1977Paper

An analysis of factors influencing the isolation rate of herpes simplex virus

Microbiology and Immunology
A TadaK Yoshino


Attempts were made to improve the rate of isolation of herpes simplex virus (HSV) from clinical specimens by minimizing loss of virus infectivity during transportation and employing the most sensitive cells for isolation. Basical analyses using standard strains of type 1 and type 2 HSV indicated that virus titer decrease was marked even at low temperatures in environments free of proteinous stabilizer such as normal serum or tissue extract, negating the generally held concept that HSV is stable in distilled water. YLE (Earle-lactalbumin HYDROLYSATE-YEAST EXTRACT) medium containing 20% inactivated calf serum was determined to be a transport medium of choice, because degradation of suspended virus during storage and freeze-thawing was negligible and loss of virus during Millipore filtration was minimal. Special coating of the membrane could also be obviated by the use of this solution. In a cell susceptibility test using clinical specimens, secondary rabbit kidney (SRK) cells were the most sensitive, showing a quick development of cytopathic effect. Vero and RK-13 cells were the second best, whereas monkey kidney, HeLa and L cells were far less sensitive. A total of 136 specimens from suspected cases, sent by dermatologists, were...Continue Reading


Oct 30, 1976·Lancet·T KawanaS Sakamoto
Feb 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·J P YangN S Chen
Jan 1, 1969·Archives of Ophthalmology·V R ColemanE Jawetz
Jun 1, 1967·Journal of Virology·C Wallis, J L Melnick
Jan 1, 1974·Canadian Journal of Microbiology·F R Bishai, N A Labzoffsky
Aug 1, 1974·Japanese Journal of Medical Science & Biology·R Hondo
Oct 1, 1953·The Journal of Experimental Medicine·H AMOS
Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT
Jun 1, 1963·The Journal of Hygiene·B LIESS, W PLOWRIGHT
Mar 13, 1964·Archiv für die gesamte Virusforschung·S TANIGUCHI, K YOSHINO

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Jan 1, 1978·Microbiology and Immunology·A Tada, K Yoshino
Jan 1, 1981·Microbiology and Immunology·K Yoshino, K Abe
Mar 1, 1989·Journal of Medical Virology·S P GuglielminoS Cianci
Feb 1, 1981·The Journal of Dermatology·F OtsukaT Shishiba
Apr 1, 1990·The Journal of Dermatology·M Yoshida, T Tezuka
Jul 1, 1982·Journal of Clinical Microbiology·N BalachandranW E Rawls
Apr 1, 1990·Clinical Microbiology Reviews·F B Johnson

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