PMID: 8583010Jul 1, 1995Paper

An analysis of multinucleated blastomere formation in human embryos

Human Reproduction
S J PickeringP R Braude

Abstract

Human embryos were disaggregated into component blastomeres 42-72 h after insemination. The blastomeres were scored for the number of nuclei present and blastomeres of known nuclear morphology were returned to individual culture drops for 16-20 h, after which they were scored for cleavage and nuclear morphology. In all, 48% of mononucleated blastomeres cleaved during this period, but only 76% of these produced two mononucleated daughter blastomeres; in the remainder, one or more of the blastomeres was abnormally nucleated. During overnight culture, 30% of multinucleated blastomeres and 30% of anucleate blastomeres cleaved, the majority producing abnormally nucleated daughter blastomeres. The majority of blastomeres which showed no sign of cleavage after overnight culture retained the same nuclear morphology as when originally disaggregated. However, a small number of mononucleated blastomeres contained two nuclei after culture, indicating that karyokinesis may have taken place in the absence of cytokinesis. Overall, approximately 30% of blastomeres with more than one nucleus seemed to arise by this mechanism, the remainder probably arising by errors of chromosome segregation and/or packaging at mitosis. In addition, 25/111 mono...Continue Reading

Citations

Oct 31, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J YangJ Norman
Dec 21, 2006·Journal of Assisted Reproduction and Genetics·Mónica GilFrancisco Rísquez
Feb 8, 2008·Journal of Assisted Reproduction and Genetics·I E AgerholmS Ziebe
Mar 31, 2012·Journal of Assisted Reproduction and Genetics·Kirstine KirkegaardHans Jakob Ingerslev
Apr 12, 2007·Reproductive Biomedicine Online·Artem ShkumatovYury Verlinsky
Apr 1, 2003·Animal Health Research Reviews·Robert P Hunter
Jun 1, 2004·Inflammatory Bowel Diseases·M LöwenbergD W Hommes
Jul 20, 2010·Reproductive Biomedicine Online·A FinnJ Hill
Sep 9, 2015·The Journal of Reproduction and Development·Akiyoshi EgashiraTakashi Kuramoto
Apr 6, 2006·Fertility and Sterility·Haydar Nadir CirayMustafa Bahçeci
Dec 21, 2014·Fertility and Sterility·Kirstine KirkegaardThorir Hardarson
Nov 25, 2006·Fertility and Sterility·M Cristina MagliValeria Farfalli
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Sep 1, 2000·Human Reproduction·J Gerris, E Van Royen
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Aug 9, 2018·Systems Biology in Reproductive Medicine·Mara SimopoulouMichael Koutsilieris
Sep 21, 2006·Prenatal Diagnosis·Jie DengGuang-Lun Zhuang

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