An analysis of plant-aphid interactions by different microarray hybridization strategies

Molecular Ecology
C VoelckelIan T Baldwin

Abstract

Aphids have long been considered 'stealthy' herbivores that subvert a plant's induced defenses and manipulate its source-sink signaling, but these hypotheses are largely untested at a transcriptional level. We analysed gene expression in native tobacco plants (Nicotiana attenuata) infested with Myzus nicotianae aphids, without resorting to the use of clip-cages, with a cDNA microarray containing 240 defense-related N. attenuata genes. Using a hybridization scheme ('ratio analysis' and 'state analysis') broadly applicable in two-factor analyses, we examined how the aphids influenced source--sink relationships and determined if their feeding preference, apart from benefiting from the sink strength of young leaves, was associated with the expression of known plant defense genes. In contrast to the responses elicited by attack from tissue-feeding lepidopteran larvae and mesophyll-sucking insects, attack from phloem-feeding aphids elicited only weak responses. Similar to other herbivores, M. nicotianae feeding increased the expression of trypsin protease inhibitors (TPI), lipoxygenase, and xyloglucan-endotransglycosylase genes, and decreased small RUBISCO subunit and ubiquitin carrier protein transcripts. Aphid-specific changes incl...Continue Reading

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Citations

Jul 10, 2009·Journal of Chemical Ecology·Murugesan RangasamyRussell T Nagata
Apr 19, 2011·Journal of Chemical Ecology·Xiang LiuCharles Michael Smith
Apr 12, 2005·Plant Molecular Biology·Fanchon DivolSylvie Dinant
Jun 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alex C C WilsonKatherine B Hurley
Jan 26, 2007·Molecular Plant-microbe Interactions : MPMI·Ling-Ling GaoKaram B Singh
Nov 6, 2007·Molecular Plant-microbe Interactions : MPMI·Natacha Bodenhausen, Philippe Reymond
Dec 7, 2007·Molecular Plant-microbe Interactions : MPMI·Yuanyuan WangGuangcun He
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Nov 17, 2012·BMC Plant Biology·Manasi MishraAshok P Giri
Mar 25, 2014·Plant Science : an International Journal of Experimental Plant Biology·Van Chung MaiIwona Morkunas
Jun 6, 2012·The Arabidopsis Book·Joe LouisJyoti Shah
Sep 6, 2007·Drug Metabolism Reviews·Tadeja RezenDamjana Rozman
Jun 15, 2010·Comptes rendus biologies·Philippe GiordanengoJean-Louis Bonnemain
Oct 1, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Sophie St-Cyr, Nadia Aubin-Horth
Feb 3, 2009·Plant, Cell & Environment·Jianqiang Wu, Ian T Baldwin
Mar 10, 2007·The Plant Journal : for Cell and Molecular Biology·Shree P Pandey, Ian T Baldwin

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