An analysis of the bias in red blood cell hemolysis measurement using several analytical approaches

Clinica Chimica Acta; International Journal of Clinical Chemistry
Jason P AckerQi-Long Yi


Percentage hemolysis in red cell concentrates (RCC) for transfusion is an indicator of RBC damage. As several factors need to be measured to determine hemolysis, and multiple assays are available for each, the choice of analytical methodology could critically influence results. Hemolysis was measured in 48 RCCs every 7 days during storage including expiry (42 days), with supernatant hemoglobin measured using the reference Drabkin's cyanmethemoglobin method and the Harboe spectrophotometric method, total hemoglobin measured using Drabkin's method and 3 automated analyzers (ADVIA 120, CELL-DYN 1700, Coulter AcT), and hematocrit measured using traditional centrifugation and automated analyzers. The choice of method affects hemolysis measurement. Biases ranging from -0.01- -0.03% were observed depending on the combination of methods used. Hematocrit measurement appeared to be a major determinant of bias, and the greatest bias was seen with the ADVIA 120 automated analyzer. Although results did not differ by a level thought to be of clinical significance, the choice of method will impact quality control pass/fail rates. Although guidelines exist in many jurisdictions regarding acceptable hemolysis levels in RCCs, these are silent re...Continue Reading


Jun 15, 1984·The Biochemical Journal·J SzebeniR W Carrell
Jan 17, 2002·The Journal of Surgical Research·Tamara L BerezinaGeorge W Machiedo
Jan 1, 1959·Scandinavian Journal of Clinical and Laboratory Investigation·M HARBOE
Aug 19, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fatime ImeriAndreas R Huber
Feb 19, 2010·Transfusion·W M Andrew Heaton
Apr 19, 2011·Transfusion·Daniel B Kim-ShapiroMark T Gladwin
Jul 1, 2007·Asian Journal of Transfusion Science·R B SawantP T Kadam

❮ Previous
Next ❯


Apr 15, 2014·Cryobiology·Mariia ZhurovaJason P Acker
Jul 10, 2013·Transfusion·Jayme D R KurachJason P Acker
Jul 6, 2016·International Journal of Experimental Pathology·Max Seidy SaitoPlínio Cunha Satlher
Sep 18, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kyla M WalterBirgit Puschner
Jan 22, 2020·The Journal of Clinical Investigation·Richard O FrancisEldad A Hod
Aug 11, 2020·Science Advances·Yongsheng GaoSamir Mitragotri
Jun 13, 2018·Laboratory Medicine·Rekha GautamRakesh P Patel
Nov 21, 2019·BMC Complementary and Alternative Medicine·Molly Mombeshora, Stanley Mukanganyama
Jan 16, 2021·International Journal of Laboratory Hematology·Emilia C CalvaresiJonathan R Genzen
Sep 17, 2020·Science Advances·Yongsheng GaoSamir Mitragotri

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.