An analysis of the bias in red blood cell hemolysis measurement using several analytical approaches

Clinica Chimica Acta; International Journal of Clinical Chemistry
Jason P AckerQi-Long Yi

Abstract

Percentage hemolysis in red cell concentrates (RCC) for transfusion is an indicator of RBC damage. As several factors need to be measured to determine hemolysis, and multiple assays are available for each, the choice of analytical methodology could critically influence results. Hemolysis was measured in 48 RCCs every 7 days during storage including expiry (42 days), with supernatant hemoglobin measured using the reference Drabkin's cyanmethemoglobin method and the Harboe spectrophotometric method, total hemoglobin measured using Drabkin's method and 3 automated analyzers (ADVIA 120, CELL-DYN 1700, Coulter AcT), and hematocrit measured using traditional centrifugation and automated analyzers. The choice of method affects hemolysis measurement. Biases ranging from -0.01- -0.03% were observed depending on the combination of methods used. Hematocrit measurement appeared to be a major determinant of bias, and the greatest bias was seen with the ADVIA 120 automated analyzer. Although results did not differ by a level thought to be of clinical significance, the choice of method will impact quality control pass/fail rates. Although guidelines exist in many jurisdictions regarding acceptable hemolysis levels in RCCs, these are silent re...Continue Reading

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Citations

Apr 15, 2014·Cryobiology·Mariia ZhurovaJason P Acker
Jul 10, 2013·Transfusion·Jayme D R KurachJason P Acker
Jul 6, 2016·International Journal of Experimental Pathology·Max Seidy SaitoPlínio Cunha Satlher
Sep 18, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kyla M WalterBirgit Puschner
Jan 22, 2020·The Journal of Clinical Investigation·Richard O FrancisEldad A Hod
Aug 11, 2020·Science Advances·Yongsheng GaoSamir Mitragotri
Jun 13, 2018·Laboratory Medicine·Rekha GautamRakesh P Patel
Nov 21, 2019·BMC Complementary and Alternative Medicine·Molly Mombeshora, Stanley Mukanganyama
Jan 16, 2021·International Journal of Laboratory Hematology·Emilia C CalvaresiJonathan R Genzen
Sep 17, 2020·Science Advances·Yongsheng GaoSamir Mitragotri

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