An analysis of the effects of retinoic acid and other retinoids on the development of adrenergic cells from the avian neural crest

Experimental Cell Research
J M Rockwood, G D Maxwell

Abstract

In the present work, we have investigated the role of all-trans-retinoic acid (all-trans RA), and several other natural and synthetic retinoids, in the development of adrenergic cells in quail neural crest cultures. Dose response studies using all-trans RA and 13-cis RA revealed a dose-dependent increase in the number of adrenergic cells in neural crest cultures. Similar dose response studies using RA isomers and other natural retinoids did not result in the same increases. In order to determine the receptor mediating the effects of all-trans RA in the neural crest, we tested several synthetic analogs which specifically bind to a particular RA receptor (RAR) subtype. We found that the compound AM 580, which activates the RAR-alpha, produced an increase in adrenergic cells similar to that seen with all-trans RA. The compound TTNPB, which activates all RAR subtypes, also resulted in an increase in adrenergic cells. We conclude that the increase in adrenergic cells seen with all-trans RA is mediated by RAR-alpha and possibly RAR-beta. To further define the actions of all-trans RA on the neural crest we incubated cultures with 5-bromo-2'-deoxyuridine (BrdU) to determine whether all-trans RA could affect the rate of proliferation. T...Continue Reading

Citations

Jun 30, 2006·Journal of Neurochemistry·Hyerhan JeongWongi Seol
Dec 25, 2004·Developmental Biology·Marthe J Howard
Dec 18, 2001·Anais Da Academia Brasileira De Ciências·E DupinN Ledouarin
May 22, 2019·Genesis : the Journal of Genetics and Development·Christof NolteRobb Krumlauf
Jul 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M Sieber-Blum
Dec 1, 2015·Polish Journal of Veterinary Sciences·N NowikJ Kaleczyc

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