An analysis of the expression of collagen I and III genes in the fascia of obese patients

The Journal of Surgical Research
Wojciech SzczesnyJuliusz Polak

Abstract

The etiology of incisional hernias in the population of morbidly obese patients remains unclear. Most likely, factors other than purely mechanical are at play; it has been ascertained that nonobese patients suffering from inguinal and incisional hernias display alterations in the architecture of the connective tissue. The goal of this study has been to evaluate and compare the relative expression of collagen type I and III genes in the rectus abdominis muscle sheath (RMS) of obese and nonobese individuals to investigate their possible influence on the quality of the connective tissue. RMS specimens were harvested in the early stages of either bariatric or non-bariatric laparotomies; total RNA was isolated and enzymatically purified from the tissue samples. The resulting material was subjected to a quantitative and qualitative analysis; reverse transcription reactions were then performed and the resulting complementary DNA was used in real-time reverse transcription polymerase chain reactions. The biopsy specimens were also examined by scanning electron microscopy. The real-time reverse transcription polymerase chain reactions, performed on complementary DNA, provided specific amplicons for individual genes. The efficacy of the ...Continue Reading

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Citations

Apr 3, 2020·Arquivos brasileiros de cardiologia·Danielle Cristina Tomaz da Silva-BertaniAntonio Carlos Cicogna
May 15, 2018·Hip International : the Journal of Clinical and Experimental Research on Hip Pathology and Therapy·Brian T NickelThorsten M Seyler
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Aug 4, 2021·Hernia : the Journal of Hernias and Abdominal Wall Surgery·M Mughal, D Ross
Apr 16, 2020·Life Sciences·Danielle Cristina Tomaz da Silva-BertaniAntonio Carlos Cicogna

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