An analysis of the heterogeneity of albumin

Preparative Biochemistry
J JanatovaJ D Andrade

Abstract

A simple and rapid procedure for characterization of serum albumin samples and for the isolation of fractions from fresh serum or commercial sources has been developed, based on DEAE Sepharose CL-6B ion exchange chromatography. Characterization of the fractions included sulfhydryl analysis, gel electrophoresis, and immunoelectrophoresis. Both analytical and preparative procedures were used. It has been shown that the relative content of the fractions varies from individual to individual. It has also been shown that certain fractions found in commercial preparations are artifacts which should be removed before performing serious studies with serum albumin.

References

Oct 1, 1977·The Biochemical Journal·S Angal, P D Dean
Aug 1, 1977·Vox Sanguinis·J M CurlingS Eriksson
Sep 1, 1976·Analytical Biochemistry·S AslamT R Brown
Apr 22, 1971·Journal of Chromatography·M CeskaF Grossmüller
Nov 4, 1974·Biochimica Et Biophysica Acta·A Wichman, L O Andersson
Jan 1, 1974·Methods in Enzymology·S Shaltiel
Jun 1, 1974·FEBS Letters·J Carlsson, A Svenson
Mar 28, 1966·Biochimica Et Biophysica Acta·L O Andersson
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Jul 1, 1947·Journal of the American Chemical Society·W L HUGHES

Related Concepts

Bos indicus
Ion-Exchange Chromatography Procedure
Plasma Albumin
Serum Albumin, Bovine

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