An Analysis of Trafficking Receptors Shows that CD44 and P-Selectin Glycoprotein Ligand-1 Collectively Control the Migration of Activated Human T-Cells

Frontiers in Immunology
Amal J AliJasmeen S Merzaban

Abstract

Selectins guide the traffic of activated T-cells through the blood stream by mediating their tethering and rolling onto inflamed endothelium, in this way acting as beacons to help navigate them to sites of inflammation. Here, we present a comprehensive analysis of E-selectin ligands expressed on activated human T-cells. We identified several novel glycoproteins that function as E-selectin ligands. Specifically, we compared the role of P-selectin glycoprotein ligand-1 (PSGL-1) and CD43, known E-selectin ligands, to CD44, a ligand that has not previously been characterized as an E-selectin ligand on activated human T-cells. We showed that CD44 acts as a functional E-selectin ligand when expressed on both CD4(+) and CD8(+) T-cells. Moreover, the CD44 protein carries a binding epitope identifying it as hematopoietic cell E- and/or L-selectin ligand (HCELL). Furthermore, by knocking down these ligands individually or together in primary activated human T-cells, we demonstrated that CD44/HCELL, and not CD43, cooperates with PSGL-1 as a major E-selectin ligand. Additionally, we demonstrated the relevance of our findings to chronic autoimmune disease, by showing that CD44/HCELL and PSGL-1, but not CD43, from T-cells isolated from psori...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G R ScreatonJ I Bell
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B ArdmanD E Staunton
Nov 21, 1991·Nature·Y RosensteinS J Burakoff
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M J PolleyJ C Paulson
Oct 12, 1995·Nature·N ManjunathB Ardman
Apr 1, 1996·Virchows Archiv : an International Journal of Pathology·A J Staal-van den BrekelE F Wouters
Apr 7, 1998·Immunity·B M StocktonU H von Andrian
Dec 8, 1998·The Journal of Experimental Medicine·R C WoodmanP Kubes
Jun 8, 1999·The Journal of Experimental Medicine·H XieA H Lichtman
Jun 13, 2001·The Journal of Cell Biology·C J DimitroffR Sackstein
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thandi M OnamiRafi Ahmed
Jan 4, 2003·Nature Reviews. Molecular Cell Biology·Helmut PontaPeter A Herrlich
Apr 19, 2003·Journal of the European Academy of Dermatology and Venereology : JEADV·J C Prinz
Jun 28, 2003·Trends in Molecular Medicine·Klaus Ley
Dec 12, 2003·Nature Reviews. Immunology·Ulrich H von Andrian, Thorsten R Mempel
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Javier Mestas, Christopher C W Hughes
May 4, 2004·Nature Reviews. Immunology·Klaus Ley, Geoffrey S Kansas
Sep 24, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Yulia VugmeysterJames G Krueger
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jasmeen S MerzabanHermann J Ziltener
Apr 13, 2005·The Journal of Experimental Medicine·Yoshio KatayamaPaul S Frenette
Jul 5, 2005·Cancer Research·William D HanleyRobert Sackstein
Nov 5, 2005·Blood·Robert C FuhlbriggeThomas S Kupper
Dec 20, 2005·Archives of Dermatological Research·Markus FriedrichGerhard Wolff
Dec 22, 2005·Nature Immunology·Andrew D LusterUlrich H von Andrian
Feb 25, 2006·Blood·Claudine S BonderPaul Kubes
Jun 27, 2006·Methods in Molecular Biology·Robert Sackstein, Robert Fuhlbrigge
Sep 8, 2006·The FEBS Journal·Markus Sperandio
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Douglas A Carlow, Hermann J Ziltener
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masanori MatsumotoTakako Hirata
Mar 27, 2009·The Journal of Investigative Dermatology·Antonella Di CesareFrank O Nestle
Apr 21, 2009·Methods in Molecular Biology·Robert Sackstein, Robert Fuhlbrigge
Dec 15, 2010·The Journal of Experimental Medicine·Zhenghui LiuRodger P McEver
Apr 5, 2011·The American Journal of Pathology·Maria NácherAndrés Hidalgo
Jun 17, 2011·Briefings in Bioinformatics·Walid H Gharib, Marc Robinson-Rechavi
Aug 2, 2011·Trends in Immunology·Irina B MazoUlrich H von Andrian

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Datasets Mentioned

BETA
GMI-1070

Methods Mentioned

BETA
flow cytometry
immunoprecipitation assay
chip
transfection
transfections
glycosylation

Software Mentioned

Imaris
FlowJo
BIAcore
GraphPad Prism

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