An ancient C-type lectin in Chlamys farreri (CfLec-2) that mediate pathogen recognition and cellular adhesion

Developmental and Comparative Immunology
Jialong YangLinsheng Song

Abstract

C-type lectins are a superfamily of Ca(2+) dependent carbohydrate-recognition proteins which play significant diverse roles in nonself-recognition and clearance of invaders. In the present study, a C-type lectin (CfLec-2) from Zhikong scallop Chlamys farreri was selected to investigate its functions in innate immunity. The mRNA expression of CfLec-2 in hemocytes was significantly up-regulated (P<0.01) after scallops were stimulated by LPS, PGN or β-glucan, and reached the highest expression level at 12h post-stimulation, which was 72.5-, 23.6- or 43.8-fold compared with blank group, respectively. The recombinant CfLec-2 (designated as rCfLec-2) could bind LPS, PGN, mannan and zymosan in vitro, but it could not bind β-glucan. Immunofluorescence assay with polyclonal antibody specific for CfLec-2 revealed that CfLec-2 was mainly located in the mantle, kidney and gonad. Furthermore, rCfLec-2 could bind to the surface of scallop hemocytes, and then initiated cellular adhesion and recruited hemocytes to enhance their encapsulation in vitro, and this process could be specifically blocked by anti-rCfLec-2 serum. These results collectively suggested that CfLec-2 from the primitive deuterostome C. farreri could perform two distinct immu...Continue Reading

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Citations

May 15, 2013·Fish & Shellfish Immunology·Jingjing WangLinsheng Song
Aug 12, 2015·Marine Drugs·Johan GardèresWerner E G Müller
Jan 2, 2013·Developmental and Comparative Immunology·Mengmeng HuangLinsheng Song
Aug 7, 2012·Developmental and Comparative Immunology·Lingling WangLinsheng Song
Nov 1, 2011·Developmental and Comparative Immunology·Leilei WangLinsheng Song
Jan 18, 2011·Developmental and Comparative Immunology·Xiaoyan SongXiaolin Liu
Jun 9, 2015·Fish & Shellfish Immunology·Xiumei WeiJialong Yang
Mar 24, 2015·Fish & Shellfish Immunology·Linsheng SongMengqiang Wang
Sep 7, 2016·Developmental and Comparative Immunology·Mengmeng HuangLinsheng Song
Mar 8, 2018·International Journal of Molecular Sciences·Weilin WangLinsheng Song
Apr 21, 2018·Archives of Insect Biochemistry and Physiology·Jing ZhouHui Ai
Feb 26, 2019·Frontiers in Physiology·Katherina BrokordtGeorgina A Rivera-Ingraham
Dec 18, 2020·Polymers·Hongtao ZhuZhaoming Dong
May 1, 2021·Developmental and Comparative Immunology·Xiaowei ChenLinsheng Song
May 9, 2021·Journal of Invertebrate Pathology·Yijing HanDinglong Yang

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