An ancient conserved gene expressed in the human inner ear: identification, expression analysis, and chromosomal mapping of human and mouse antiquitin (ATQ1)

Genomics
A B SkvorakC C Morton

Abstract

We constructed and screened a human fetal cochlear cDNA library to identify genes involved in hearing and deafness. From this library we isolated a cDNA corresponding to the highly conserved ancient gene antiquitin (ATQ1). The plant homolog of ATQ1 is thought to be involved in regulating turgor pressure, a function that also would be essential for cells of the mammalian cochlea. Northern blots of 13 human fetal tissues show antiquitin to be highly expressed in cochlea, ovary, eye, heart, and kidney. Using RT-PCR of rat cochlear hair cell-specific cDNA libraries, we detect antiquitin expression in outer hair cells, but not in inner or vestibular type 1 hair cells, suggesting that antiquitin is not expressed ubiquitously in the cochlea. Human ATQ1 was mapped to human chromosome region 5q31 using fluorescence in situ hybridization, and mouse ATQ1 was mapped to mouse chromosome 18 by single-strand conformation polymorphism mapping of interspecific backcross progeny DNAs. Four human antiquitin-like sequences, possibly pseudogenes, were also identified and mapped.

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P E LeonM C King
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J EberwineP Coleman
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R N Van GelderJ H Eberwine
Jan 1, 1991·Annals of the New York Academy of Sciences·K P Steel
Apr 1, 1990·Ear and Hearing·W E Brownell
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 5, 1989·Nature·A J Hudspeth
Jan 11, 1985·Science·W E BrownellY de Ribaupierre
Feb 1, 1995·Plant Molecular Biology·V L StroeherA G Good
Nov 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D R Beier
Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·K F Manly
May 1, 1996·Genomics·R W DeBry, M F Seldin

❮ Previous
Next ❯

Citations

Sep 11, 2004·Journal of the Association for Research in Otolaryngology : JARO·Barbara L ResendesCynthia C Morton
Jun 27, 2002·Journal of the Association for Research in Otolaryngology : JARO·Barbara L ResendesCynthia C Morton
Jul 9, 2004·Drug Metabolism Reviews·Vasilis VasiliouTia Estey
Jul 10, 2008·Expert Opinion on Drug Metabolism & Toxicology·Satori A MarchittiVasilis Vasiliou
Aug 16, 2002·The Journal of Physiology·Stefan Heller
Mar 5, 2003·Journal of Biochemical and Molecular Toxicology·Norman E Sládek
Jul 13, 2002·American Journal of Medical Genetics·Michael LynchBrian C Pyman
Sep 2, 2009·Brain Research Bulletin·Dimitrios StagosVasilis Vasiliou
Feb 16, 2007·Hearing Research·Michael S HildebrandHans-Henrik M Dahl
Feb 27, 2003·Chemico-biological Interactions·Wing-Ping FongWai-Kwan Tang
Oct 17, 2002·Journal of Neurobiology·Stefan Heller
Jul 2, 2004·Biology of Reproduction·Zdenka EllederovaHana Kovarova
Nov 14, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yazen Alnouti, Curtis D Klaassen
Jun 10, 2006·Journal of Inherited Metabolic Disease·Peter T Clayton
Mar 9, 2010·The Journal of Biological Chemistry·Chad BrockerVasilis Vasiliou
Jul 21, 2016·Metabolic Brain Disease·Florent MarguetSoumeya Bekri
Jan 13, 2001·Chemico-biological Interactions·V VasiliouD R Petersen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.